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Circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria

Natural coastal microbial mat communities are multi-species assemblages that experience fluctuating environmental conditions and are shaped by resource competition as well as by cooperation. Laboratory studies rarely address the natural complexity of microbial communities but are usually limited to...

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Autores principales: Hörnlein, Christine, Confurius-Guns, Veronique, Grego, Michele, Stal, Lucas J., Bolhuis, Henk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445270/
https://www.ncbi.nlm.nih.gov/pubmed/32839512
http://dx.doi.org/10.1038/s41598-020-69294-3
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author Hörnlein, Christine
Confurius-Guns, Veronique
Grego, Michele
Stal, Lucas J.
Bolhuis, Henk
author_facet Hörnlein, Christine
Confurius-Guns, Veronique
Grego, Michele
Stal, Lucas J.
Bolhuis, Henk
author_sort Hörnlein, Christine
collection PubMed
description Natural coastal microbial mat communities are multi-species assemblages that experience fluctuating environmental conditions and are shaped by resource competition as well as by cooperation. Laboratory studies rarely address the natural complexity of microbial communities but are usually limited to homogeneous mono-cultures of key species grown in liquid media. The mat-forming filamentous cyanobacteria Lyngbya aestuarii and Coleofasciculus chthonoplastes were cultured under different conditions to investigate the expression of circadian clock genes and genes that are under their control. The cyanobacteria were grown in liquid medium or on a solid substrate (glass beads) as mono- or as co-cultures under a light–dark regime and subsequently transferred to continuous light. TaqMan-probe based qPCR assays were used to quantify the expression of the circadian clock genes kaiA, kaiB, and kaiC, and of four genes that are under control of the circadian clock: psbA, nifH, ftsZ, and prx. Expression of kaiABC was influenced by co-culturing the cyanobacteria and whether grown in liquid media or on a solid substrate. Free-running (i.e. under continuous light) expression cycle of the circadian clock genes was observed in L. aestuarii but not in C. chthonoplastes. In the former organism, maximum expression of psbA and nifH occurred temporally separated and independent of the light regime, although the peak shifted in time when the culture was transferred to continuous illumination. Although functionally similar, both species of cyanobacteria displayed different 24-h transcriptional patterns in response to the experimental treatments, suggesting that their circadian clocks have adapted to different life strategies adopted by these mat-forming cyanobacteria.
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spelling pubmed-74452702020-08-26 Circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria Hörnlein, Christine Confurius-Guns, Veronique Grego, Michele Stal, Lucas J. Bolhuis, Henk Sci Rep Article Natural coastal microbial mat communities are multi-species assemblages that experience fluctuating environmental conditions and are shaped by resource competition as well as by cooperation. Laboratory studies rarely address the natural complexity of microbial communities but are usually limited to homogeneous mono-cultures of key species grown in liquid media. The mat-forming filamentous cyanobacteria Lyngbya aestuarii and Coleofasciculus chthonoplastes were cultured under different conditions to investigate the expression of circadian clock genes and genes that are under their control. The cyanobacteria were grown in liquid medium or on a solid substrate (glass beads) as mono- or as co-cultures under a light–dark regime and subsequently transferred to continuous light. TaqMan-probe based qPCR assays were used to quantify the expression of the circadian clock genes kaiA, kaiB, and kaiC, and of four genes that are under control of the circadian clock: psbA, nifH, ftsZ, and prx. Expression of kaiABC was influenced by co-culturing the cyanobacteria and whether grown in liquid media or on a solid substrate. Free-running (i.e. under continuous light) expression cycle of the circadian clock genes was observed in L. aestuarii but not in C. chthonoplastes. In the former organism, maximum expression of psbA and nifH occurred temporally separated and independent of the light regime, although the peak shifted in time when the culture was transferred to continuous illumination. Although functionally similar, both species of cyanobacteria displayed different 24-h transcriptional patterns in response to the experimental treatments, suggesting that their circadian clocks have adapted to different life strategies adopted by these mat-forming cyanobacteria. Nature Publishing Group UK 2020-08-24 /pmc/articles/PMC7445270/ /pubmed/32839512 http://dx.doi.org/10.1038/s41598-020-69294-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hörnlein, Christine
Confurius-Guns, Veronique
Grego, Michele
Stal, Lucas J.
Bolhuis, Henk
Circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria
title Circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria
title_full Circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria
title_fullStr Circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria
title_full_unstemmed Circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria
title_short Circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria
title_sort circadian clock-controlled gene expression in co-cultured, mat-forming cyanobacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445270/
https://www.ncbi.nlm.nih.gov/pubmed/32839512
http://dx.doi.org/10.1038/s41598-020-69294-3
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