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iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae

PURPOSE: Treatment of infections with Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases (ESBLs) is challenging due to the coexistence of multiple resistance mechanisms and the hypervirulent variant. Therefore, new targets or more effective treatment options aimed at ESBL-prod...

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Detalles Bibliográficos
Autores principales: Wang, Yan, Cong, Shan, Zhang, Qinghua, Li, Ranwei, Wang, Ke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445504/
https://www.ncbi.nlm.nih.gov/pubmed/32903891
http://dx.doi.org/10.2147/IDR.S259894
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author Wang, Yan
Cong, Shan
Zhang, Qinghua
Li, Ranwei
Wang, Ke
author_facet Wang, Yan
Cong, Shan
Zhang, Qinghua
Li, Ranwei
Wang, Ke
author_sort Wang, Yan
collection PubMed
description PURPOSE: Treatment of infections with Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases (ESBLs) is challenging due to the coexistence of multiple resistance mechanisms and the hypervirulent variant. Therefore, new targets or more effective treatment options aimed at ESBL-producing Klebsiella pneumoniae are urgently needed. MATERIALS AND METHODS: Here, we collected ESBL-producing and non-ESBL Klebsiella pneumoniae isolates and studied their differences from a proteomic point of view. RESULTS: We revealed treA, wza, gnd, rmlA, rmlC, rmlD, galE, aceE, and sucD as important virulence-related proteins in ESBL-producing Klebsiella pneumoniae, distinct from those in non-ESBL strains. CONCLUSION: Our findings provide plausible anti-virulence targets and suggest new therapeutic avenues against ESBL-producing Klebsiella pneumoniae.
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spelling pubmed-74455042020-09-04 iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae Wang, Yan Cong, Shan Zhang, Qinghua Li, Ranwei Wang, Ke Infect Drug Resist Original Research PURPOSE: Treatment of infections with Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases (ESBLs) is challenging due to the coexistence of multiple resistance mechanisms and the hypervirulent variant. Therefore, new targets or more effective treatment options aimed at ESBL-producing Klebsiella pneumoniae are urgently needed. MATERIALS AND METHODS: Here, we collected ESBL-producing and non-ESBL Klebsiella pneumoniae isolates and studied their differences from a proteomic point of view. RESULTS: We revealed treA, wza, gnd, rmlA, rmlC, rmlD, galE, aceE, and sucD as important virulence-related proteins in ESBL-producing Klebsiella pneumoniae, distinct from those in non-ESBL strains. CONCLUSION: Our findings provide plausible anti-virulence targets and suggest new therapeutic avenues against ESBL-producing Klebsiella pneumoniae. Dove 2020-08-19 /pmc/articles/PMC7445504/ /pubmed/32903891 http://dx.doi.org/10.2147/IDR.S259894 Text en © 2020 Wang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wang, Yan
Cong, Shan
Zhang, Qinghua
Li, Ranwei
Wang, Ke
iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae
title iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae
title_full iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae
title_fullStr iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae
title_full_unstemmed iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae
title_short iTRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing Klebsiella pneumoniae
title_sort itraq-based proteomics reveals potential anti-virulence targets for esbl-producing klebsiella pneumoniae
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445504/
https://www.ncbi.nlm.nih.gov/pubmed/32903891
http://dx.doi.org/10.2147/IDR.S259894
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