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A set of experimentally validated, mutually orthogonal primers for combinatorially specifying genetic components

The design and synthesis of novel genes and deoxyribonucleic acid (DNA) sequences is a central technique in synthetic biology. Current methods of high throughput gene synthesis use pooled oligonucleotides obtained from custom-designed DNA microarray chips, and rely on orthogonal (non-interacting) po...

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Detalles Bibliográficos
Autores principales: Subramanian, Subu K, Russ, William P, Ranganathan, Rama
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445780/
https://www.ncbi.nlm.nih.gov/pubmed/32995509
http://dx.doi.org/10.1093/synbio/ysx008