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A set of experimentally validated, mutually orthogonal primers for combinatorially specifying genetic components
The design and synthesis of novel genes and deoxyribonucleic acid (DNA) sequences is a central technique in synthetic biology. Current methods of high throughput gene synthesis use pooled oligonucleotides obtained from custom-designed DNA microarray chips, and rely on orthogonal (non-interacting) po...
Autores principales: | Subramanian, Subu K, Russ, William P, Ranganathan, Rama |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445780/ https://www.ncbi.nlm.nih.gov/pubmed/32995509 http://dx.doi.org/10.1093/synbio/ysx008 |
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