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Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery

Extracellular vesicles (EVs) in human blood are a potential source of biomarkers. To which extent anticoagulation affects their concentration, cellular origin and protein composition is largely unexplored. To study this, blood from 23 healthy subjects was collected in acid citrate dextrose (ACD), ci...

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Autores principales: Palviainen, Mari, Saraswat, Mayank, Varga, Zoltán, Kitka, Diána, Neuvonen, Maarit, Puhka, Maija, Joenväärä, Sakari, Renkonen, Risto, Nieuwland, Rienk, Takatalo, Maarit, Siljander, Pia R. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7446890/
https://www.ncbi.nlm.nih.gov/pubmed/32813744
http://dx.doi.org/10.1371/journal.pone.0236439
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author Palviainen, Mari
Saraswat, Mayank
Varga, Zoltán
Kitka, Diána
Neuvonen, Maarit
Puhka, Maija
Joenväärä, Sakari
Renkonen, Risto
Nieuwland, Rienk
Takatalo, Maarit
Siljander, Pia R. M.
author_facet Palviainen, Mari
Saraswat, Mayank
Varga, Zoltán
Kitka, Diána
Neuvonen, Maarit
Puhka, Maija
Joenväärä, Sakari
Renkonen, Risto
Nieuwland, Rienk
Takatalo, Maarit
Siljander, Pia R. M.
author_sort Palviainen, Mari
collection PubMed
description Extracellular vesicles (EVs) in human blood are a potential source of biomarkers. To which extent anticoagulation affects their concentration, cellular origin and protein composition is largely unexplored. To study this, blood from 23 healthy subjects was collected in acid citrate dextrose (ACD), citrate or EDTA, or without anticoagulation to obtain serum. EVs were isolated by ultracentrifugation or by size-exclusion chromatography (SEC) for fluorescence-SEC. EVs were analyzed by micro flow cytometry, NTA, TEM, Western blot, and protein mass spectrometry. The plasma EV concentration was unaffected by anticoagulants, but serum contained more platelet EVs. The protein composition of plasma EVs differed between anticoagulants, and between plasma and serum. Comparison to other studies further revealed that the shared EV protein composition resembles the “protein corona” of synthetic nanoparticles incubated in plasma or serum. In conclusion, we have validated a higher concentration of platelet EVs in serum than plasma by contemporary EV methods. Anticoagulation should be carefully described (i) to enable study comparison, (ii) to utilize available sample cohorts, and (iii) when preparing/selecting biobank samples. Further, the similarity of the EV protein corona and that of nanoparticles implicates that EVs carry both intravesicular and extravesicular cargo, which will expand their applicability for biomarker discovery.
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spelling pubmed-74468902020-08-26 Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery Palviainen, Mari Saraswat, Mayank Varga, Zoltán Kitka, Diána Neuvonen, Maarit Puhka, Maija Joenväärä, Sakari Renkonen, Risto Nieuwland, Rienk Takatalo, Maarit Siljander, Pia R. M. PLoS One Research Article Extracellular vesicles (EVs) in human blood are a potential source of biomarkers. To which extent anticoagulation affects their concentration, cellular origin and protein composition is largely unexplored. To study this, blood from 23 healthy subjects was collected in acid citrate dextrose (ACD), citrate or EDTA, or without anticoagulation to obtain serum. EVs were isolated by ultracentrifugation or by size-exclusion chromatography (SEC) for fluorescence-SEC. EVs were analyzed by micro flow cytometry, NTA, TEM, Western blot, and protein mass spectrometry. The plasma EV concentration was unaffected by anticoagulants, but serum contained more platelet EVs. The protein composition of plasma EVs differed between anticoagulants, and between plasma and serum. Comparison to other studies further revealed that the shared EV protein composition resembles the “protein corona” of synthetic nanoparticles incubated in plasma or serum. In conclusion, we have validated a higher concentration of platelet EVs in serum than plasma by contemporary EV methods. Anticoagulation should be carefully described (i) to enable study comparison, (ii) to utilize available sample cohorts, and (iii) when preparing/selecting biobank samples. Further, the similarity of the EV protein corona and that of nanoparticles implicates that EVs carry both intravesicular and extravesicular cargo, which will expand their applicability for biomarker discovery. Public Library of Science 2020-08-19 /pmc/articles/PMC7446890/ /pubmed/32813744 http://dx.doi.org/10.1371/journal.pone.0236439 Text en © 2020 Palviainen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Palviainen, Mari
Saraswat, Mayank
Varga, Zoltán
Kitka, Diána
Neuvonen, Maarit
Puhka, Maija
Joenväärä, Sakari
Renkonen, Risto
Nieuwland, Rienk
Takatalo, Maarit
Siljander, Pia R. M.
Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery
title Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery
title_full Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery
title_fullStr Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery
title_full_unstemmed Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery
title_short Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—Implications for biomarker discovery
title_sort extracellular vesicles from human plasma and serum are carriers of extravesicular cargo—implications for biomarker discovery
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7446890/
https://www.ncbi.nlm.nih.gov/pubmed/32813744
http://dx.doi.org/10.1371/journal.pone.0236439
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