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Association between serum anti-ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension

The aim of the present study was to identify novel antibody markers for the early diagnosis of atherosclerosis in order to improve the prognosis of patients at risk for acute ischemic stroke (AIS) and acute myocardial infarction (AMI). A first screening involved the serological identification of ant...

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Autores principales: Li, Shu-Yang, Yoshida, Yoichi, Kobayashi, Eiichi, Adachi, Akihiko, Hirono, Seiichiro, Matsutani, Tomoo, Mine, Seiichiro, Machida, Toshio, Ohno, Mikiko, Nishi, Eiichiro, Maezawa, Yoshiro, Takemoto, Minoru, Yokote, Koutaro, Kitamura, Kenichiro, Sumazaki, Makoto, Ito, Masaaki, Shimada, Hideaki, Takizawa, Hirotaka, Kashiwado, Koichi, Tomiyoshi, Go, Shinmen, Natsuko, Nakamura, Rika, Kuroda, Hideyuki, Zhang, Xiao-Meng, Wang, Hao, Goto, Kenichiro, Iwadate, Yasuo, Hiwasa, Takaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447314/
https://www.ncbi.nlm.nih.gov/pubmed/32945427
http://dx.doi.org/10.3892/ijmm.2020.4690
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author Li, Shu-Yang
Yoshida, Yoichi
Kobayashi, Eiichi
Adachi, Akihiko
Hirono, Seiichiro
Matsutani, Tomoo
Mine, Seiichiro
Machida, Toshio
Ohno, Mikiko
Nishi, Eiichiro
Maezawa, Yoshiro
Takemoto, Minoru
Yokote, Koutaro
Kitamura, Kenichiro
Sumazaki, Makoto
Ito, Masaaki
Shimada, Hideaki
Takizawa, Hirotaka
Kashiwado, Koichi
Tomiyoshi, Go
Shinmen, Natsuko
Nakamura, Rika
Kuroda, Hideyuki
Zhang, Xiao-Meng
Wang, Hao
Goto, Kenichiro
Iwadate, Yasuo
Hiwasa, Takaki
author_facet Li, Shu-Yang
Yoshida, Yoichi
Kobayashi, Eiichi
Adachi, Akihiko
Hirono, Seiichiro
Matsutani, Tomoo
Mine, Seiichiro
Machida, Toshio
Ohno, Mikiko
Nishi, Eiichiro
Maezawa, Yoshiro
Takemoto, Minoru
Yokote, Koutaro
Kitamura, Kenichiro
Sumazaki, Makoto
Ito, Masaaki
Shimada, Hideaki
Takizawa, Hirotaka
Kashiwado, Koichi
Tomiyoshi, Go
Shinmen, Natsuko
Nakamura, Rika
Kuroda, Hideyuki
Zhang, Xiao-Meng
Wang, Hao
Goto, Kenichiro
Iwadate, Yasuo
Hiwasa, Takaki
author_sort Li, Shu-Yang
collection PubMed
description The aim of the present study was to identify novel antibody markers for the early diagnosis of atherosclerosis in order to improve the prognosis of patients at risk for acute ischemic stroke (AIS) and acute myocardial infarction (AMI). A first screening involved the serological identification of antigens by recombinant cDNA expression cloning and identified additional sex combs-like 2 (ASXL2) as a target antigen recognized by serum IgG antibodies in the sera of patients with atherosclerosis. Antigens, including the recombinant glutathione S-transferasefused ASXL2 protein and its synthetic peptide were then prepared to examine serum antibody levels. Amplified luminescence proximity homoge-neous assay-linked immunosorbent assay, which incorporates glutathione-donor beads and anti-human-IgG-acceptor beads, revealed significantly higher serum antibody levels against the ASXL2 protein and its peptide in the patients with AIS, diabetes mellitus, AMI, chronic kidney disease, esophageal squamous cell carcinoma, or colorectal carcinoma compared with those in healthy donors. The ASXL2 antibody levels were well associated with hypertension complication, but not with sex, body mass index, habitual smoking, or alcohol intake. These results suggest that the serum ASXL2 antibody marker can discriminate between hypertension-induced atherosclerotic AIS and AMI, as well as a number of digestive organ cancers.
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spelling pubmed-74473142020-08-28 Association between serum anti-ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension Li, Shu-Yang Yoshida, Yoichi Kobayashi, Eiichi Adachi, Akihiko Hirono, Seiichiro Matsutani, Tomoo Mine, Seiichiro Machida, Toshio Ohno, Mikiko Nishi, Eiichiro Maezawa, Yoshiro Takemoto, Minoru Yokote, Koutaro Kitamura, Kenichiro Sumazaki, Makoto Ito, Masaaki Shimada, Hideaki Takizawa, Hirotaka Kashiwado, Koichi Tomiyoshi, Go Shinmen, Natsuko Nakamura, Rika Kuroda, Hideyuki Zhang, Xiao-Meng Wang, Hao Goto, Kenichiro Iwadate, Yasuo Hiwasa, Takaki Int J Mol Med Articles The aim of the present study was to identify novel antibody markers for the early diagnosis of atherosclerosis in order to improve the prognosis of patients at risk for acute ischemic stroke (AIS) and acute myocardial infarction (AMI). A first screening involved the serological identification of antigens by recombinant cDNA expression cloning and identified additional sex combs-like 2 (ASXL2) as a target antigen recognized by serum IgG antibodies in the sera of patients with atherosclerosis. Antigens, including the recombinant glutathione S-transferasefused ASXL2 protein and its synthetic peptide were then prepared to examine serum antibody levels. Amplified luminescence proximity homoge-neous assay-linked immunosorbent assay, which incorporates glutathione-donor beads and anti-human-IgG-acceptor beads, revealed significantly higher serum antibody levels against the ASXL2 protein and its peptide in the patients with AIS, diabetes mellitus, AMI, chronic kidney disease, esophageal squamous cell carcinoma, or colorectal carcinoma compared with those in healthy donors. The ASXL2 antibody levels were well associated with hypertension complication, but not with sex, body mass index, habitual smoking, or alcohol intake. These results suggest that the serum ASXL2 antibody marker can discriminate between hypertension-induced atherosclerotic AIS and AMI, as well as a number of digestive organ cancers. D.A. Spandidos 2020-10 2020-07-29 /pmc/articles/PMC7447314/ /pubmed/32945427 http://dx.doi.org/10.3892/ijmm.2020.4690 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Shu-Yang
Yoshida, Yoichi
Kobayashi, Eiichi
Adachi, Akihiko
Hirono, Seiichiro
Matsutani, Tomoo
Mine, Seiichiro
Machida, Toshio
Ohno, Mikiko
Nishi, Eiichiro
Maezawa, Yoshiro
Takemoto, Minoru
Yokote, Koutaro
Kitamura, Kenichiro
Sumazaki, Makoto
Ito, Masaaki
Shimada, Hideaki
Takizawa, Hirotaka
Kashiwado, Koichi
Tomiyoshi, Go
Shinmen, Natsuko
Nakamura, Rika
Kuroda, Hideyuki
Zhang, Xiao-Meng
Wang, Hao
Goto, Kenichiro
Iwadate, Yasuo
Hiwasa, Takaki
Association between serum anti-ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension
title Association between serum anti-ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension
title_full Association between serum anti-ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension
title_fullStr Association between serum anti-ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension
title_full_unstemmed Association between serum anti-ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension
title_short Association between serum anti-ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension
title_sort association between serum anti-asxl2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447314/
https://www.ncbi.nlm.nih.gov/pubmed/32945427
http://dx.doi.org/10.3892/ijmm.2020.4690
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