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A two-lane mechanism for selective biological ammonium transport

The transport of charged molecules across biological membranes faces the dual problem of accommodating charges in a highly hydrophobic environment while maintaining selective substrate translocation. This has been the subject of a particular controversy for the exchange of ammonium across cellular m...

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Autores principales: Williamson, Gordon, Tamburrino, Giulia, Bizior, Adriana, Boeckstaens, Mélanie, Dias Mirandela, Gaëtan, Bage, Marcus G, Pisliakov, Andrei, Ives, Callum M, Terras, Eilidh, Hoskisson, Paul A, Marini, Anna Maria, Zachariae, Ulrich, Javelle, Arnaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447429/
https://www.ncbi.nlm.nih.gov/pubmed/32662768
http://dx.doi.org/10.7554/eLife.57183
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author Williamson, Gordon
Tamburrino, Giulia
Bizior, Adriana
Boeckstaens, Mélanie
Dias Mirandela, Gaëtan
Bage, Marcus G
Pisliakov, Andrei
Ives, Callum M
Terras, Eilidh
Hoskisson, Paul A
Marini, Anna Maria
Zachariae, Ulrich
Javelle, Arnaud
author_facet Williamson, Gordon
Tamburrino, Giulia
Bizior, Adriana
Boeckstaens, Mélanie
Dias Mirandela, Gaëtan
Bage, Marcus G
Pisliakov, Andrei
Ives, Callum M
Terras, Eilidh
Hoskisson, Paul A
Marini, Anna Maria
Zachariae, Ulrich
Javelle, Arnaud
author_sort Williamson, Gordon
collection PubMed
description The transport of charged molecules across biological membranes faces the dual problem of accommodating charges in a highly hydrophobic environment while maintaining selective substrate translocation. This has been the subject of a particular controversy for the exchange of ammonium across cellular membranes, an essential process in all domains of life. Ammonium transport is mediated by the ubiquitous Amt/Mep/Rh transporters that includes the human Rhesus factors. Here, using a combination of electrophysiology, yeast functional complementation and extended molecular dynamics simulations, we reveal a unique two-lane pathway for electrogenic NH(4)(+) transport in two archetypal members of the family, the transporters AmtB from Escherichia coli and Rh50 from Nitrosomonas europaea. The pathway underpins a mechanism by which charged H(+) and neutral NH(3) are carried separately across the membrane after NH(4)(+) deprotonation. This mechanism defines a new principle of achieving transport selectivity against competing ions in a biological transport process.
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spelling pubmed-74474292020-08-27 A two-lane mechanism for selective biological ammonium transport Williamson, Gordon Tamburrino, Giulia Bizior, Adriana Boeckstaens, Mélanie Dias Mirandela, Gaëtan Bage, Marcus G Pisliakov, Andrei Ives, Callum M Terras, Eilidh Hoskisson, Paul A Marini, Anna Maria Zachariae, Ulrich Javelle, Arnaud eLife Biochemistry and Chemical Biology The transport of charged molecules across biological membranes faces the dual problem of accommodating charges in a highly hydrophobic environment while maintaining selective substrate translocation. This has been the subject of a particular controversy for the exchange of ammonium across cellular membranes, an essential process in all domains of life. Ammonium transport is mediated by the ubiquitous Amt/Mep/Rh transporters that includes the human Rhesus factors. Here, using a combination of electrophysiology, yeast functional complementation and extended molecular dynamics simulations, we reveal a unique two-lane pathway for electrogenic NH(4)(+) transport in two archetypal members of the family, the transporters AmtB from Escherichia coli and Rh50 from Nitrosomonas europaea. The pathway underpins a mechanism by which charged H(+) and neutral NH(3) are carried separately across the membrane after NH(4)(+) deprotonation. This mechanism defines a new principle of achieving transport selectivity against competing ions in a biological transport process. eLife Sciences Publications, Ltd 2020-07-14 /pmc/articles/PMC7447429/ /pubmed/32662768 http://dx.doi.org/10.7554/eLife.57183 Text en © 2020, Williamson et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Williamson, Gordon
Tamburrino, Giulia
Bizior, Adriana
Boeckstaens, Mélanie
Dias Mirandela, Gaëtan
Bage, Marcus G
Pisliakov, Andrei
Ives, Callum M
Terras, Eilidh
Hoskisson, Paul A
Marini, Anna Maria
Zachariae, Ulrich
Javelle, Arnaud
A two-lane mechanism for selective biological ammonium transport
title A two-lane mechanism for selective biological ammonium transport
title_full A two-lane mechanism for selective biological ammonium transport
title_fullStr A two-lane mechanism for selective biological ammonium transport
title_full_unstemmed A two-lane mechanism for selective biological ammonium transport
title_short A two-lane mechanism for selective biological ammonium transport
title_sort two-lane mechanism for selective biological ammonium transport
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447429/
https://www.ncbi.nlm.nih.gov/pubmed/32662768
http://dx.doi.org/10.7554/eLife.57183
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