Cargando…

Microparticles expressing myeloperoxidase as potential biomarkers in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV)

ABSTRACT: To investigate presence of circulating myeloperoxidase-positive microparticles (MPO(+)MPs) in relation to disease activity in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Forty-six patients with AAV and 23 age- and sex-matched healthy controls were...

Descripción completa

Detalles Bibliográficos
Autores principales: Manojlovic, M., Juto, A., Jonasdottir, A., Colic, J., Vojinovic, J., Nordin, A., Bruchfeld, A., Gunnarsson, I., Mobarrez, F., Antovic, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447662/
https://www.ncbi.nlm.nih.gov/pubmed/32734361
http://dx.doi.org/10.1007/s00109-020-01955-2
_version_ 1783574348733349888
author Manojlovic, M.
Juto, A.
Jonasdottir, A.
Colic, J.
Vojinovic, J.
Nordin, A.
Bruchfeld, A.
Gunnarsson, I.
Mobarrez, F.
Antovic, A.
author_facet Manojlovic, M.
Juto, A.
Jonasdottir, A.
Colic, J.
Vojinovic, J.
Nordin, A.
Bruchfeld, A.
Gunnarsson, I.
Mobarrez, F.
Antovic, A.
author_sort Manojlovic, M.
collection PubMed
description ABSTRACT: To investigate presence of circulating myeloperoxidase-positive microparticles (MPO(+)MPs) in relation to disease activity in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Forty-six patients with AAV and 23 age- and sex-matched healthy controls were included. Vasculitis disease activity was assessed using the Birmingham Vasculitis Activity Score (BVAS). MPs were analyzed in citrate plasma by flow cytometry and phenotyped based on MPO expression and co-expression of pentraxin-3 (PTX3), high mobility group box 1 protein (HMGB1), and tumor necrosis factor-like weak inducer of apoptosis (TWEAK). Serum levels of PTX3, sTWEAK, and HMGB1 were also determined. Twenty-three patients had active vasculitis (BVAS ≥ 1). Concentrations of MPO(+)MPs expressing PTX3, HMGB1, and TWEAK were significantly higher in patients compared to healthy controls (p < 0.001, p < 0.01, p < 0.001, respectively), while concentrations of PTX3(+) and HMGB1(+)MPO(+)MPs were significantly higher in active AAV compared to patients in remission. MPO(+)MPs expressing either PTX3 or HMGB1 were associated with BVAS (r = 0.5, p < 0.001; r = 0.3, p = 0.04, respectively). Significantly higher serum PTX3 levels were found in active- than in inactive AAV (p < 0.001), correlating strongly with BVAS (r = 0.7, p < 0.001). Serum levels of sTWEAK and HMGB1 did not differ between patients and controls. Concentration of MPO(+)MPs is increased in plasma from AAV patients compared to healthy individuals. PTX3 in serum as well as PTX3 and HMGB1 expressed on MPO(+)MPs were associated with disease activity in the investigated patients. KEY MESSAGES: Myeloperoxidase-positive microparticles (MPO(+)MPs) are increased in plasma from patients with ANCA-associated vasculitis. Concentrations of MPO(+)MPs expressing PTX3, HMGB1, and TWEAK were significantly higher in patients compared to healthy controls. MPO(+)MPs expressing PTX3 and HMGB1 are associated with disease activity in ANCA-associated vasculitis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00109-020-01955-2) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-7447662
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-74476622020-09-02 Microparticles expressing myeloperoxidase as potential biomarkers in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV) Manojlovic, M. Juto, A. Jonasdottir, A. Colic, J. Vojinovic, J. Nordin, A. Bruchfeld, A. Gunnarsson, I. Mobarrez, F. Antovic, A. J Mol Med (Berl) Original Article ABSTRACT: To investigate presence of circulating myeloperoxidase-positive microparticles (MPO(+)MPs) in relation to disease activity in patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Forty-six patients with AAV and 23 age- and sex-matched healthy controls were included. Vasculitis disease activity was assessed using the Birmingham Vasculitis Activity Score (BVAS). MPs were analyzed in citrate plasma by flow cytometry and phenotyped based on MPO expression and co-expression of pentraxin-3 (PTX3), high mobility group box 1 protein (HMGB1), and tumor necrosis factor-like weak inducer of apoptosis (TWEAK). Serum levels of PTX3, sTWEAK, and HMGB1 were also determined. Twenty-three patients had active vasculitis (BVAS ≥ 1). Concentrations of MPO(+)MPs expressing PTX3, HMGB1, and TWEAK were significantly higher in patients compared to healthy controls (p < 0.001, p < 0.01, p < 0.001, respectively), while concentrations of PTX3(+) and HMGB1(+)MPO(+)MPs were significantly higher in active AAV compared to patients in remission. MPO(+)MPs expressing either PTX3 or HMGB1 were associated with BVAS (r = 0.5, p < 0.001; r = 0.3, p = 0.04, respectively). Significantly higher serum PTX3 levels were found in active- than in inactive AAV (p < 0.001), correlating strongly with BVAS (r = 0.7, p < 0.001). Serum levels of sTWEAK and HMGB1 did not differ between patients and controls. Concentration of MPO(+)MPs is increased in plasma from AAV patients compared to healthy individuals. PTX3 in serum as well as PTX3 and HMGB1 expressed on MPO(+)MPs were associated with disease activity in the investigated patients. KEY MESSAGES: Myeloperoxidase-positive microparticles (MPO(+)MPs) are increased in plasma from patients with ANCA-associated vasculitis. Concentrations of MPO(+)MPs expressing PTX3, HMGB1, and TWEAK were significantly higher in patients compared to healthy controls. MPO(+)MPs expressing PTX3 and HMGB1 are associated with disease activity in ANCA-associated vasculitis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00109-020-01955-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-07-30 2020 /pmc/articles/PMC7447662/ /pubmed/32734361 http://dx.doi.org/10.1007/s00109-020-01955-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Manojlovic, M.
Juto, A.
Jonasdottir, A.
Colic, J.
Vojinovic, J.
Nordin, A.
Bruchfeld, A.
Gunnarsson, I.
Mobarrez, F.
Antovic, A.
Microparticles expressing myeloperoxidase as potential biomarkers in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV)
title Microparticles expressing myeloperoxidase as potential biomarkers in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV)
title_full Microparticles expressing myeloperoxidase as potential biomarkers in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV)
title_fullStr Microparticles expressing myeloperoxidase as potential biomarkers in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV)
title_full_unstemmed Microparticles expressing myeloperoxidase as potential biomarkers in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV)
title_short Microparticles expressing myeloperoxidase as potential biomarkers in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV)
title_sort microparticles expressing myeloperoxidase as potential biomarkers in anti-neutrophil cytoplasmic antibody (anca)-associated vasculitides (aav)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447662/
https://www.ncbi.nlm.nih.gov/pubmed/32734361
http://dx.doi.org/10.1007/s00109-020-01955-2
work_keys_str_mv AT manojlovicm microparticlesexpressingmyeloperoxidaseaspotentialbiomarkersinantineutrophilcytoplasmicantibodyancaassociatedvasculitidesaav
AT jutoa microparticlesexpressingmyeloperoxidaseaspotentialbiomarkersinantineutrophilcytoplasmicantibodyancaassociatedvasculitidesaav
AT jonasdottira microparticlesexpressingmyeloperoxidaseaspotentialbiomarkersinantineutrophilcytoplasmicantibodyancaassociatedvasculitidesaav
AT colicj microparticlesexpressingmyeloperoxidaseaspotentialbiomarkersinantineutrophilcytoplasmicantibodyancaassociatedvasculitidesaav
AT vojinovicj microparticlesexpressingmyeloperoxidaseaspotentialbiomarkersinantineutrophilcytoplasmicantibodyancaassociatedvasculitidesaav
AT nordina microparticlesexpressingmyeloperoxidaseaspotentialbiomarkersinantineutrophilcytoplasmicantibodyancaassociatedvasculitidesaav
AT bruchfelda microparticlesexpressingmyeloperoxidaseaspotentialbiomarkersinantineutrophilcytoplasmicantibodyancaassociatedvasculitidesaav
AT gunnarssoni microparticlesexpressingmyeloperoxidaseaspotentialbiomarkersinantineutrophilcytoplasmicantibodyancaassociatedvasculitidesaav
AT mobarrezf microparticlesexpressingmyeloperoxidaseaspotentialbiomarkersinantineutrophilcytoplasmicantibodyancaassociatedvasculitidesaav
AT antovica microparticlesexpressingmyeloperoxidaseaspotentialbiomarkersinantineutrophilcytoplasmicantibodyancaassociatedvasculitidesaav