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FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides

FvatfA from the maize pathogen Fusarium verticillioides putatively encodes the Aspergillus nidulans AtfA and Schizasaccharomyces pombe Atf1 orthologous bZIP-type transcription factor, FvAtfA. In this study, a ΔFvatfA deletion mutant was constructed and then genetically complemented with the fully fu...

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Autores principales: Szabó, Zsuzsa, Pákozdi, Klaudia, Murvai, Katalin, Pusztahelyi, Tünde, Kecskeméti, Ádám, Gáspár, Attila, Logrieco, Antonio F., Emri, Tamás, Ádám, Attila L., Leiter, Éva, Hornok, László, Pócsi, István
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447684/
https://www.ncbi.nlm.nih.gov/pubmed/32719911
http://dx.doi.org/10.1007/s00253-020-10717-6
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author Szabó, Zsuzsa
Pákozdi, Klaudia
Murvai, Katalin
Pusztahelyi, Tünde
Kecskeméti, Ádám
Gáspár, Attila
Logrieco, Antonio F.
Emri, Tamás
Ádám, Attila L.
Leiter, Éva
Hornok, László
Pócsi, István
author_facet Szabó, Zsuzsa
Pákozdi, Klaudia
Murvai, Katalin
Pusztahelyi, Tünde
Kecskeméti, Ádám
Gáspár, Attila
Logrieco, Antonio F.
Emri, Tamás
Ádám, Attila L.
Leiter, Éva
Hornok, László
Pócsi, István
author_sort Szabó, Zsuzsa
collection PubMed
description FvatfA from the maize pathogen Fusarium verticillioides putatively encodes the Aspergillus nidulans AtfA and Schizasaccharomyces pombe Atf1 orthologous bZIP-type transcription factor, FvAtfA. In this study, a ΔFvatfA deletion mutant was constructed and then genetically complemented with the fully functional FvatfA gene. Comparing phenotypic features of the wild-type parental, the deletion mutant and the restored strains shed light on the versatile regulatory functions played by FvAtfA in (i) the maintenance of vegetative growth on Czapek-Dox and Potato Dextrose agars and invasive growth on unwounded tomato fruits, (ii) the preservation of conidiospore yield and size, (iii) the orchestration of oxidative (H(2)O(2), menadione sodium bisulphite) and cell wall integrity (Congo Red) stress defences and (iv) the regulation of mycotoxin (fumonisins) and pigment (bikaverin, carotenoid) productions. Expression of selected biosynthetic genes both in the fumonisin (fum1, fum8) and the carotenoid (carRA, carB) pathways were down-regulated in the ΔFvatfA strain resulting in defected fumonisin production and considerably decreased carotenoid yields. The expression of bik1, encoding the polyketide synthase needed in bikaverin biosynthesis, was not up-regulated by the deletion of FvatfA meanwhile the ΔFvatfA strain produced approximately ten times more bikaverin than the wild-type or the genetically complemented strains. The abolishment of fumonisin production of the ΔFvatfA strain may lead to the development of new-type, biology-based mycotoxin control strategies. The novel information gained on the regulation of pigment production by this fungus can be interesting for experts working on new, Fusarium-based biomass and pigment production technologies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00253-020-10717-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-74476842020-09-02 FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides Szabó, Zsuzsa Pákozdi, Klaudia Murvai, Katalin Pusztahelyi, Tünde Kecskeméti, Ádám Gáspár, Attila Logrieco, Antonio F. Emri, Tamás Ádám, Attila L. Leiter, Éva Hornok, László Pócsi, István Appl Microbiol Biotechnol Applied Genetics and Molecular Biotechnology FvatfA from the maize pathogen Fusarium verticillioides putatively encodes the Aspergillus nidulans AtfA and Schizasaccharomyces pombe Atf1 orthologous bZIP-type transcription factor, FvAtfA. In this study, a ΔFvatfA deletion mutant was constructed and then genetically complemented with the fully functional FvatfA gene. Comparing phenotypic features of the wild-type parental, the deletion mutant and the restored strains shed light on the versatile regulatory functions played by FvAtfA in (i) the maintenance of vegetative growth on Czapek-Dox and Potato Dextrose agars and invasive growth on unwounded tomato fruits, (ii) the preservation of conidiospore yield and size, (iii) the orchestration of oxidative (H(2)O(2), menadione sodium bisulphite) and cell wall integrity (Congo Red) stress defences and (iv) the regulation of mycotoxin (fumonisins) and pigment (bikaverin, carotenoid) productions. Expression of selected biosynthetic genes both in the fumonisin (fum1, fum8) and the carotenoid (carRA, carB) pathways were down-regulated in the ΔFvatfA strain resulting in defected fumonisin production and considerably decreased carotenoid yields. The expression of bik1, encoding the polyketide synthase needed in bikaverin biosynthesis, was not up-regulated by the deletion of FvatfA meanwhile the ΔFvatfA strain produced approximately ten times more bikaverin than the wild-type or the genetically complemented strains. The abolishment of fumonisin production of the ΔFvatfA strain may lead to the development of new-type, biology-based mycotoxin control strategies. The novel information gained on the regulation of pigment production by this fungus can be interesting for experts working on new, Fusarium-based biomass and pigment production technologies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00253-020-10717-6) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-07-27 2020 /pmc/articles/PMC7447684/ /pubmed/32719911 http://dx.doi.org/10.1007/s00253-020-10717-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Applied Genetics and Molecular Biotechnology
Szabó, Zsuzsa
Pákozdi, Klaudia
Murvai, Katalin
Pusztahelyi, Tünde
Kecskeméti, Ádám
Gáspár, Attila
Logrieco, Antonio F.
Emri, Tamás
Ádám, Attila L.
Leiter, Éva
Hornok, László
Pócsi, István
FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides
title FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides
title_full FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides
title_fullStr FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides
title_full_unstemmed FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides
title_short FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides
title_sort fvatfa regulates growth, stress tolerance as well as mycotoxin and pigment productions in fusarium verticillioides
topic Applied Genetics and Molecular Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447684/
https://www.ncbi.nlm.nih.gov/pubmed/32719911
http://dx.doi.org/10.1007/s00253-020-10717-6
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