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Immunogenicity assessment of Clostridium perfringens type D epsilon toxin epitope-based chimeric construct in mice and rabbit

Epsilon toxin (Etx) belongs to family of pore-forming toxin and is produced by Clostridium perfringens type D. The Etx toxin is responsible for the pathogenesis of enterotoxaemia in sheep and goats, and occasionally in other livestock animals. The present study aimed to develop a Clostridium perfrin...

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Autores principales: Singh, Ajay Pratap, Prabhu, Shyama N., Nagaleekar, Viswas K., Dangi, Saroj K., Prakash, Chandan, Singh, Vijendra Pal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447850/
https://www.ncbi.nlm.nih.gov/pubmed/32864287
http://dx.doi.org/10.1007/s13205-020-02400-4
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author Singh, Ajay Pratap
Prabhu, Shyama N.
Nagaleekar, Viswas K.
Dangi, Saroj K.
Prakash, Chandan
Singh, Vijendra Pal
author_facet Singh, Ajay Pratap
Prabhu, Shyama N.
Nagaleekar, Viswas K.
Dangi, Saroj K.
Prakash, Chandan
Singh, Vijendra Pal
author_sort Singh, Ajay Pratap
collection PubMed
description Epsilon toxin (Etx) belongs to family of pore-forming toxin and is produced by Clostridium perfringens type D. The Etx toxin is responsible for the pathogenesis of enterotoxaemia in sheep and goats, and occasionally in other livestock animals. The present study aimed to develop a Clostridium perfringens epsilon toxin-based chimeric epitope construct having immunodominant B-cell epitope and universal T-cell epitope and its immunogenicity was evaluated in mice and rabbit. An artificial chimeric epitope construct (CEC) was prepared by joining tandem repeats of a peptide containing amino acids (aa) 134–145 of epsilon toxin B-cell epitope and universal T-cell epitopes. The CEC was expressed in the Escherichia coli following codon optimization for efficient translational efficiency and purified by affinity chromatography. The antigenic reactivity of r-CEC proteins was confirmed by western blot with rabbit anti-r-Etox hyperimmune sera. The immunogenicity of the recombinant single CEC was examined in mice and rabbit by indirect ELISA. It was found that r-CEC yielded high titers of neutralizing antibodies (≥ 1.035 IU/ml) in immunized mice and rabbit. The potency of chimeric protein immunized serum was observed to be higher than the recommended level (0.1–0.3 IU/ml) for protection in sheep and goats. This indicated the potential ability of the chimeric protein as a vaccine candidate. This further requires studying the immune response in targeted host species (sheep and goat).
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spelling pubmed-74478502020-08-26 Immunogenicity assessment of Clostridium perfringens type D epsilon toxin epitope-based chimeric construct in mice and rabbit Singh, Ajay Pratap Prabhu, Shyama N. Nagaleekar, Viswas K. Dangi, Saroj K. Prakash, Chandan Singh, Vijendra Pal 3 Biotech Original Article Epsilon toxin (Etx) belongs to family of pore-forming toxin and is produced by Clostridium perfringens type D. The Etx toxin is responsible for the pathogenesis of enterotoxaemia in sheep and goats, and occasionally in other livestock animals. The present study aimed to develop a Clostridium perfringens epsilon toxin-based chimeric epitope construct having immunodominant B-cell epitope and universal T-cell epitope and its immunogenicity was evaluated in mice and rabbit. An artificial chimeric epitope construct (CEC) was prepared by joining tandem repeats of a peptide containing amino acids (aa) 134–145 of epsilon toxin B-cell epitope and universal T-cell epitopes. The CEC was expressed in the Escherichia coli following codon optimization for efficient translational efficiency and purified by affinity chromatography. The antigenic reactivity of r-CEC proteins was confirmed by western blot with rabbit anti-r-Etox hyperimmune sera. The immunogenicity of the recombinant single CEC was examined in mice and rabbit by indirect ELISA. It was found that r-CEC yielded high titers of neutralizing antibodies (≥ 1.035 IU/ml) in immunized mice and rabbit. The potency of chimeric protein immunized serum was observed to be higher than the recommended level (0.1–0.3 IU/ml) for protection in sheep and goats. This indicated the potential ability of the chimeric protein as a vaccine candidate. This further requires studying the immune response in targeted host species (sheep and goat). Springer International Publishing 2020-08-26 2020-09 /pmc/articles/PMC7447850/ /pubmed/32864287 http://dx.doi.org/10.1007/s13205-020-02400-4 Text en © King Abdulaziz City for Science and Technology 2020
spellingShingle Original Article
Singh, Ajay Pratap
Prabhu, Shyama N.
Nagaleekar, Viswas K.
Dangi, Saroj K.
Prakash, Chandan
Singh, Vijendra Pal
Immunogenicity assessment of Clostridium perfringens type D epsilon toxin epitope-based chimeric construct in mice and rabbit
title Immunogenicity assessment of Clostridium perfringens type D epsilon toxin epitope-based chimeric construct in mice and rabbit
title_full Immunogenicity assessment of Clostridium perfringens type D epsilon toxin epitope-based chimeric construct in mice and rabbit
title_fullStr Immunogenicity assessment of Clostridium perfringens type D epsilon toxin epitope-based chimeric construct in mice and rabbit
title_full_unstemmed Immunogenicity assessment of Clostridium perfringens type D epsilon toxin epitope-based chimeric construct in mice and rabbit
title_short Immunogenicity assessment of Clostridium perfringens type D epsilon toxin epitope-based chimeric construct in mice and rabbit
title_sort immunogenicity assessment of clostridium perfringens type d epsilon toxin epitope-based chimeric construct in mice and rabbit
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447850/
https://www.ncbi.nlm.nih.gov/pubmed/32864287
http://dx.doi.org/10.1007/s13205-020-02400-4
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