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Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension

Pulmonary hypertension is a chronic vascular disease characterized by pulmonary vasoconstriction and pulmonary arterial remodeling. Pulmonary arterial remodeling is mainly due to small pulmonary arterial wall thickening and lumen occlusion. Previous studies have described intravascular changes in lu...

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Autores principales: Zhu, Zhongkai, Wang, Yifan, Long, Amy, Feng, Tianyu, Ocampo, Maria, Chen, Sunny, Tang, Haiyang, Guo, Qiang, Minshall, Richard, Makino, Ayako, Huang, Wei, Chen, Jiwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448137/
https://www.ncbi.nlm.nih.gov/pubmed/32922742
http://dx.doi.org/10.1177/2045894020922129
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author Zhu, Zhongkai
Wang, Yifan
Long, Amy
Feng, Tianyu
Ocampo, Maria
Chen, Sunny
Tang, Haiyang
Guo, Qiang
Minshall, Richard
Makino, Ayako
Huang, Wei
Chen, Jiwang
author_facet Zhu, Zhongkai
Wang, Yifan
Long, Amy
Feng, Tianyu
Ocampo, Maria
Chen, Sunny
Tang, Haiyang
Guo, Qiang
Minshall, Richard
Makino, Ayako
Huang, Wei
Chen, Jiwang
author_sort Zhu, Zhongkai
collection PubMed
description Pulmonary hypertension is a chronic vascular disease characterized by pulmonary vasoconstriction and pulmonary arterial remodeling. Pulmonary arterial remodeling is mainly due to small pulmonary arterial wall thickening and lumen occlusion. Previous studies have described intravascular changes in lung sections using histopathology, but few were able to obtain a fine detailed image of the pulmonary vascular system. In this study, we used Microfil compounds to cast the pulmonary arteries in a rat model of monocrotaline-induced pulmonary hypertension. High-quality images that enabled quantification of distal pulmonary arterial branching based on the number of vessel bifurcations/junctions were demonstrated in this model. The branch and junction counts of distal pulmonary arteries significantly decreased in the monocrotaline group compared to the control group, and this effect was inversely proportional to the mean pulmonary artery pressure observed in each group. The patterns of pulmonary vasculature and the methods for pulmonary vessel casting are presented to provide a basis for future studies of pulmonary arterial remodeling due to pulmonary hypertension and other lung diseases that involve the remodeling of vasculature.
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spelling pubmed-74481372020-09-10 Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension Zhu, Zhongkai Wang, Yifan Long, Amy Feng, Tianyu Ocampo, Maria Chen, Sunny Tang, Haiyang Guo, Qiang Minshall, Richard Makino, Ayako Huang, Wei Chen, Jiwang Pulm Circ Original Research Pulmonary hypertension is a chronic vascular disease characterized by pulmonary vasoconstriction and pulmonary arterial remodeling. Pulmonary arterial remodeling is mainly due to small pulmonary arterial wall thickening and lumen occlusion. Previous studies have described intravascular changes in lung sections using histopathology, but few were able to obtain a fine detailed image of the pulmonary vascular system. In this study, we used Microfil compounds to cast the pulmonary arteries in a rat model of monocrotaline-induced pulmonary hypertension. High-quality images that enabled quantification of distal pulmonary arterial branching based on the number of vessel bifurcations/junctions were demonstrated in this model. The branch and junction counts of distal pulmonary arteries significantly decreased in the monocrotaline group compared to the control group, and this effect was inversely proportional to the mean pulmonary artery pressure observed in each group. The patterns of pulmonary vasculature and the methods for pulmonary vessel casting are presented to provide a basis for future studies of pulmonary arterial remodeling due to pulmonary hypertension and other lung diseases that involve the remodeling of vasculature. SAGE Publications 2020-08-24 /pmc/articles/PMC7448137/ /pubmed/32922742 http://dx.doi.org/10.1177/2045894020922129 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
Zhu, Zhongkai
Wang, Yifan
Long, Amy
Feng, Tianyu
Ocampo, Maria
Chen, Sunny
Tang, Haiyang
Guo, Qiang
Minshall, Richard
Makino, Ayako
Huang, Wei
Chen, Jiwang
Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension
title Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension
title_full Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension
title_fullStr Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension
title_full_unstemmed Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension
title_short Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension
title_sort pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448137/
https://www.ncbi.nlm.nih.gov/pubmed/32922742
http://dx.doi.org/10.1177/2045894020922129
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