Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay

BACKGROUND: The burden of non-tuberculous mycobacterial (NTM) disease is increasing worldwide but still its diagnosis is delayed and it is mistaken as multidrug-resistant tuberculosis (MDR-TB).The present study was performed to develop a multiplex PCR assay for detection and identification of clinic...

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Autores principales: Singh, Kamal, Kumari, Richa, Tripathi, Rajneesh, Gupta, Smita, Anupurba, Shampa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448335/
https://www.ncbi.nlm.nih.gov/pubmed/32847517
http://dx.doi.org/10.1186/s12866-020-01952-y
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author Singh, Kamal
Kumari, Richa
Tripathi, Rajneesh
Gupta, Smita
Anupurba, Shampa
author_facet Singh, Kamal
Kumari, Richa
Tripathi, Rajneesh
Gupta, Smita
Anupurba, Shampa
author_sort Singh, Kamal
collection PubMed
description BACKGROUND: The burden of non-tuberculous mycobacterial (NTM) disease is increasing worldwide but still its diagnosis is delayed and it is mistaken as multidrug-resistant tuberculosis (MDR-TB).The present study was performed to develop a multiplex PCR assay for detection and identification of clinically most common NTM to the species level from pulmonary samples. RESULTS: Out of 50 isolates, 26 were identified as Mycobacterium kansasii (MK), 20 were identified as Mycobacterium abscessus (MA) and 4 were identified as Mycobacterium avium complex (MAC) through multiplex PCR and further confirmed by sequencing. CONCLUSION: Our study showed that multiplex PCR assay is a simple, convenient, and reliable technique for detection and differential identification of major NTM species.
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spelling pubmed-74483352020-08-27 Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay Singh, Kamal Kumari, Richa Tripathi, Rajneesh Gupta, Smita Anupurba, Shampa BMC Microbiol Research Article BACKGROUND: The burden of non-tuberculous mycobacterial (NTM) disease is increasing worldwide but still its diagnosis is delayed and it is mistaken as multidrug-resistant tuberculosis (MDR-TB).The present study was performed to develop a multiplex PCR assay for detection and identification of clinically most common NTM to the species level from pulmonary samples. RESULTS: Out of 50 isolates, 26 were identified as Mycobacterium kansasii (MK), 20 were identified as Mycobacterium abscessus (MA) and 4 were identified as Mycobacterium avium complex (MAC) through multiplex PCR and further confirmed by sequencing. CONCLUSION: Our study showed that multiplex PCR assay is a simple, convenient, and reliable technique for detection and differential identification of major NTM species. BioMed Central 2020-08-26 /pmc/articles/PMC7448335/ /pubmed/32847517 http://dx.doi.org/10.1186/s12866-020-01952-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Singh, Kamal
Kumari, Richa
Tripathi, Rajneesh
Gupta, Smita
Anupurba, Shampa
Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay
title Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay
title_full Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay
title_fullStr Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay
title_full_unstemmed Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay
title_short Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay
title_sort detection of clinically important non tuberculous mycobacteria (ntm) from pulmonary samples through one-step multiplex pcr assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448335/
https://www.ncbi.nlm.nih.gov/pubmed/32847517
http://dx.doi.org/10.1186/s12866-020-01952-y
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