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Cyto-biological effects of microRNA-424-5p on human colorectal cancer cells

MicroRNA (miR)-424-5p is overexpressed in colorectal cancer (CRC); however, its role, clinical significance and underlying molecular mechanism have remained to be fully elucidated. The aim of the present study was to investigate the roles of miR-424-5p in CRC and the underlying mechanisms. It was de...

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Autores principales: Yan, Weitao, Jiang, Xia, Wang, Guiqi, Li, Wei, Zhai, Congjie, Chen, Shihao, Shang, Fangjian, Zhao, Zengren, Yu, Weifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448566/
https://www.ncbi.nlm.nih.gov/pubmed/32863933
http://dx.doi.org/10.3892/ol.2020.11982
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author Yan, Weitao
Jiang, Xia
Wang, Guiqi
Li, Wei
Zhai, Congjie
Chen, Shihao
Shang, Fangjian
Zhao, Zengren
Yu, Weifang
author_facet Yan, Weitao
Jiang, Xia
Wang, Guiqi
Li, Wei
Zhai, Congjie
Chen, Shihao
Shang, Fangjian
Zhao, Zengren
Yu, Weifang
author_sort Yan, Weitao
collection PubMed
description MicroRNA (miR)-424-5p is overexpressed in colorectal cancer (CRC); however, its role, clinical significance and underlying molecular mechanism have remained to be fully elucidated. The aim of the present study was to investigate the roles of miR-424-5p in CRC and the underlying mechanisms. It was demonstrated that miR-424-5p is overexpressed in CRC, based on bioinformatics analysis using The Cancer Genome Atlas TCGA and analysis of tissue samples from patients with CRC from The First Hospital of Hebei Medical University, and the expression of miR-424-5p was associated with the depth of invasion and Dukes' staging. In CRC cells, the oncogenic roles of miR-424-5p were also verified by Cell Counting Kit-8, wound healing and Transwell assays. To identify target genes, all transcripts were compared between miR-424-5p mimic-transfected SW480 cells and mimic control cells by transcriptome sequencing. Subsequently, the differentially expressed genes (DEGs) were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The DEGs were revealed to be significantly enriched in the GO terms ‘serine hydrolase activity,’ ‘serine-type peptidase activity’ and ‘serine-type endopeptidase activity’. KEGG signaling pathway analysis indicated that the DEGs were significantly enriched in ‘endocytosis’, ‘regulation of actin cytoskeleton’, ‘Wnt signaling pathway’ and ‘ubiquitin-mediated proteolysis signaling pathway’. These results suggested that miR-424-5p is a potential target in the treatment of CRC.
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spelling pubmed-74485662020-08-28 Cyto-biological effects of microRNA-424-5p on human colorectal cancer cells Yan, Weitao Jiang, Xia Wang, Guiqi Li, Wei Zhai, Congjie Chen, Shihao Shang, Fangjian Zhao, Zengren Yu, Weifang Oncol Lett Articles MicroRNA (miR)-424-5p is overexpressed in colorectal cancer (CRC); however, its role, clinical significance and underlying molecular mechanism have remained to be fully elucidated. The aim of the present study was to investigate the roles of miR-424-5p in CRC and the underlying mechanisms. It was demonstrated that miR-424-5p is overexpressed in CRC, based on bioinformatics analysis using The Cancer Genome Atlas TCGA and analysis of tissue samples from patients with CRC from The First Hospital of Hebei Medical University, and the expression of miR-424-5p was associated with the depth of invasion and Dukes' staging. In CRC cells, the oncogenic roles of miR-424-5p were also verified by Cell Counting Kit-8, wound healing and Transwell assays. To identify target genes, all transcripts were compared between miR-424-5p mimic-transfected SW480 cells and mimic control cells by transcriptome sequencing. Subsequently, the differentially expressed genes (DEGs) were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The DEGs were revealed to be significantly enriched in the GO terms ‘serine hydrolase activity,’ ‘serine-type peptidase activity’ and ‘serine-type endopeptidase activity’. KEGG signaling pathway analysis indicated that the DEGs were significantly enriched in ‘endocytosis’, ‘regulation of actin cytoskeleton’, ‘Wnt signaling pathway’ and ‘ubiquitin-mediated proteolysis signaling pathway’. These results suggested that miR-424-5p is a potential target in the treatment of CRC. D.A. Spandidos 2020-10 2020-08-13 /pmc/articles/PMC7448566/ /pubmed/32863933 http://dx.doi.org/10.3892/ol.2020.11982 Text en Copyright: © Yan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yan, Weitao
Jiang, Xia
Wang, Guiqi
Li, Wei
Zhai, Congjie
Chen, Shihao
Shang, Fangjian
Zhao, Zengren
Yu, Weifang
Cyto-biological effects of microRNA-424-5p on human colorectal cancer cells
title Cyto-biological effects of microRNA-424-5p on human colorectal cancer cells
title_full Cyto-biological effects of microRNA-424-5p on human colorectal cancer cells
title_fullStr Cyto-biological effects of microRNA-424-5p on human colorectal cancer cells
title_full_unstemmed Cyto-biological effects of microRNA-424-5p on human colorectal cancer cells
title_short Cyto-biological effects of microRNA-424-5p on human colorectal cancer cells
title_sort cyto-biological effects of microrna-424-5p on human colorectal cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448566/
https://www.ncbi.nlm.nih.gov/pubmed/32863933
http://dx.doi.org/10.3892/ol.2020.11982
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