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Rapid and Sensitive Direct Detection and Identification of Poliovirus from Stool and Environmental Surveillance Samples by Use of Nanopore Sequencing

Global poliovirus surveillance involves virus isolation from stool and environmental samples, intratypic differential (ITD) by PCR, and sequencing of the VP1 region to distinguish vaccine (Sabin), vaccine-derived, and wild-type polioviruses and to ensure an appropriate response. This cell culture al...

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Detalles Bibliográficos
Autores principales: Shaw, Alexander G., Majumdar, Manasi, Troman, Catherine, O’Toole, Áine, Benny, Blossom, Abraham, Dilip, Praharaj, Ira, Kang, Gagandeep, Sharif, Salmaan, Alam, Muhammad Masroor, Shaukat, Shahzad, Angez, Mehar, Khurshid, Adnan, Mahmood, Nayab, Arshad, Yasir, Rehman, Lubna, Mujtaba, Ghulam, Akthar, Ribqa, Salman, Muhammad, Klapsa, Dimitra, Hajarha, Yara, Asghar, Humayun, Bandyopadhyay, Ananda, Rambaut, Andrew, Martin, Javier, Grassly, Nicholas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448630/
https://www.ncbi.nlm.nih.gov/pubmed/32611795
http://dx.doi.org/10.1128/JCM.00920-20