A drug screening toolkit based on the –1 ribosomal frameshifting of SARS-CoV-2

The –1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to successfully develop a toolkit for...

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Detalles Bibliográficos
Autores principales: Chen, Yanqiong, Tao, Huan, Shen, Silan, Miao, Zhiyong, Li, Lili, Jia, Yongqian, Zhang, Hu, Bai, Xiufeng, Fu, Xinyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448739/
https://www.ncbi.nlm.nih.gov/pubmed/32869005
http://dx.doi.org/10.1016/j.heliyon.2020.e04793
Descripción
Sumario:The –1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to successfully develop a toolkit for screening antiviral drugs. Finally, the FDA-approved drug library was screened, revealing that ivacaftor and (–)-Huperzine A worked well in changing the –1 ribosomal frameshifting of SARS-CoV-2 in vitro.

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