A drug screening toolkit based on the –1 ribosomal frameshifting of SARS-CoV-2

The –1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to successfully develop a toolkit for...

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Autores principales: Chen, Yanqiong, Tao, Huan, Shen, Silan, Miao, Zhiyong, Li, Lili, Jia, Yongqian, Zhang, Hu, Bai, Xiufeng, Fu, Xinyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448739/
https://www.ncbi.nlm.nih.gov/pubmed/32869005
http://dx.doi.org/10.1016/j.heliyon.2020.e04793
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author Chen, Yanqiong
Tao, Huan
Shen, Silan
Miao, Zhiyong
Li, Lili
Jia, Yongqian
Zhang, Hu
Bai, Xiufeng
Fu, Xinyuan
author_facet Chen, Yanqiong
Tao, Huan
Shen, Silan
Miao, Zhiyong
Li, Lili
Jia, Yongqian
Zhang, Hu
Bai, Xiufeng
Fu, Xinyuan
author_sort Chen, Yanqiong
collection PubMed
description The –1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to successfully develop a toolkit for screening antiviral drugs. Finally, the FDA-approved drug library was screened, revealing that ivacaftor and (–)-Huperzine A worked well in changing the –1 ribosomal frameshifting of SARS-CoV-2 in vitro.
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spelling pubmed-74487392020-08-27 A drug screening toolkit based on the –1 ribosomal frameshifting of SARS-CoV-2 Chen, Yanqiong Tao, Huan Shen, Silan Miao, Zhiyong Li, Lili Jia, Yongqian Zhang, Hu Bai, Xiufeng Fu, Xinyuan Heliyon Article The –1 ribosomal frameshifting is vital for the translation of the open reading frame (ORF)1b in SARS-CoV-2. The products of ORF1b participate in viral replication. Therefore, changing the frameshift frequency reduces the survival of the virus. This study aimed to successfully develop a toolkit for screening antiviral drugs. Finally, the FDA-approved drug library was screened, revealing that ivacaftor and (–)-Huperzine A worked well in changing the –1 ribosomal frameshifting of SARS-CoV-2 in vitro. Elsevier 2020-08-26 /pmc/articles/PMC7448739/ /pubmed/32869005 http://dx.doi.org/10.1016/j.heliyon.2020.e04793 Text en © 2020 The Authors. Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Chen, Yanqiong
Tao, Huan
Shen, Silan
Miao, Zhiyong
Li, Lili
Jia, Yongqian
Zhang, Hu
Bai, Xiufeng
Fu, Xinyuan
A drug screening toolkit based on the –1 ribosomal frameshifting of SARS-CoV-2
title A drug screening toolkit based on the –1 ribosomal frameshifting of SARS-CoV-2
title_full A drug screening toolkit based on the –1 ribosomal frameshifting of SARS-CoV-2
title_fullStr A drug screening toolkit based on the –1 ribosomal frameshifting of SARS-CoV-2
title_full_unstemmed A drug screening toolkit based on the –1 ribosomal frameshifting of SARS-CoV-2
title_short A drug screening toolkit based on the –1 ribosomal frameshifting of SARS-CoV-2
title_sort drug screening toolkit based on the –1 ribosomal frameshifting of sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7448739/
https://www.ncbi.nlm.nih.gov/pubmed/32869005
http://dx.doi.org/10.1016/j.heliyon.2020.e04793
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