A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo

Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity in vivo. Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm u...

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Detalles Bibliográficos
Autores principales: Li, Yuyao, Song, Heng, Xue, Chenghong, Fang, Zhijun, Xiong, Liqin, Xie, Hexin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7449546/
https://www.ncbi.nlm.nih.gov/pubmed/32874510
http://dx.doi.org/10.1039/d0sc01273d
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author Li, Yuyao
Song, Heng
Xue, Chenghong
Fang, Zhijun
Xiong, Liqin
Xie, Hexin
author_facet Li, Yuyao
Song, Heng
Xue, Chenghong
Fang, Zhijun
Xiong, Liqin
Xie, Hexin
author_sort Li, Yuyao
collection PubMed
description Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity in vivo. Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm upon enzymatic stimulus, significantly boosting detection sensitivity in a highly dynamic in vivo system.
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spelling pubmed-74495462020-08-31 A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo Li, Yuyao Song, Heng Xue, Chenghong Fang, Zhijun Xiong, Liqin Xie, Hexin Chem Sci Chemistry Reported herein is a self-immobilizing near-infrared fluorogenic probe that can be used to image extracellular enzyme activity in vivo. Using a fluorophore as a quinone methide precursor, this probe covalently anchors at sites of activation and greatly enhances the fluorescence intensity at 710 nm upon enzymatic stimulus, significantly boosting detection sensitivity in a highly dynamic in vivo system. Royal Society of Chemistry 2020-05-13 /pmc/articles/PMC7449546/ /pubmed/32874510 http://dx.doi.org/10.1039/d0sc01273d Text en This journal is © The Royal Society of Chemistry 2020 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0)
spellingShingle Chemistry
Li, Yuyao
Song, Heng
Xue, Chenghong
Fang, Zhijun
Xiong, Liqin
Xie, Hexin
A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo
title A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo
title_full A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo
title_fullStr A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo
title_full_unstemmed A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo
title_short A self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo
title_sort self-immobilizing near-infrared fluorogenic probe for sensitive imaging of extracellular enzyme activity in vivo
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7449546/
https://www.ncbi.nlm.nih.gov/pubmed/32874510
http://dx.doi.org/10.1039/d0sc01273d
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