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Viral loads in throat and anal swabs in children infected with SARS-CoV-2

Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay on anal swabs was recently reported to be persistently positive even after throat testing was negative during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, data about the consistent performanc...

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Autores principales: Yuan, Chunhui, Zhu, Hongmin, Yang, Yuan, Cai, Xiaonan, Xiang, Feiyan, Wu, Huan, Yao, Cong, Xiang, Yun, Xiao, Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450259/
https://www.ncbi.nlm.nih.gov/pubmed/32419639
http://dx.doi.org/10.1080/22221751.2020.1771219
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author Yuan, Chunhui
Zhu, Hongmin
Yang, Yuan
Cai, Xiaonan
Xiang, Feiyan
Wu, Huan
Yao, Cong
Xiang, Yun
Xiao, Han
author_facet Yuan, Chunhui
Zhu, Hongmin
Yang, Yuan
Cai, Xiaonan
Xiang, Feiyan
Wu, Huan
Yao, Cong
Xiang, Yun
Xiao, Han
author_sort Yuan, Chunhui
collection PubMed
description Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay on anal swabs was recently reported to be persistently positive even after throat testing was negative during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, data about the consistent performance of RT-PCR assay on throat and anal swabs remain limited in paediatric patients. Here, we retrospectively reviewed RT-PCR-testing results of 212 paediatric patients with suspected SARS-CoV-2 infection at Wuhan Children’s Hospital. The diagnostic potential of these two types of specimens showed significant difference (positive rate: 78.2% on throat swabs vs. 52.6% on anal swabs, McNemar Test P = 0.0091) and exhibited a weak positive consistency (Kappa value was 0.311, P < 0.0001) in paediatric patients. Furthermore, viral loads detected on both throat and anal swabs also showed no significant difference (P = 0.9511) and correlation (Pearson r = 0.0434, P = 0.8406), and exhibited an inconsistent kinetic change through the course of SARS-CoV-2 infection. Besides, viral loads in the throat and anal swabs were correlated with different types of immune states, immune-reactive phase, and the resolution phase/immunologic tolerance, respectively. These findings revealed that RT-PCR-testing on throat and anal swabs showed significant difference for monitoring SARS-CoV-2 infection and correlated with different immune state in paediatric patients.
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spelling pubmed-74502592020-08-31 Viral loads in throat and anal swabs in children infected with SARS-CoV-2 Yuan, Chunhui Zhu, Hongmin Yang, Yuan Cai, Xiaonan Xiang, Feiyan Wu, Huan Yao, Cong Xiang, Yun Xiao, Han Emerg Microbes Infect Articles Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay on anal swabs was recently reported to be persistently positive even after throat testing was negative during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, data about the consistent performance of RT-PCR assay on throat and anal swabs remain limited in paediatric patients. Here, we retrospectively reviewed RT-PCR-testing results of 212 paediatric patients with suspected SARS-CoV-2 infection at Wuhan Children’s Hospital. The diagnostic potential of these two types of specimens showed significant difference (positive rate: 78.2% on throat swabs vs. 52.6% on anal swabs, McNemar Test P = 0.0091) and exhibited a weak positive consistency (Kappa value was 0.311, P < 0.0001) in paediatric patients. Furthermore, viral loads detected on both throat and anal swabs also showed no significant difference (P = 0.9511) and correlation (Pearson r = 0.0434, P = 0.8406), and exhibited an inconsistent kinetic change through the course of SARS-CoV-2 infection. Besides, viral loads in the throat and anal swabs were correlated with different types of immune states, immune-reactive phase, and the resolution phase/immunologic tolerance, respectively. These findings revealed that RT-PCR-testing on throat and anal swabs showed significant difference for monitoring SARS-CoV-2 infection and correlated with different immune state in paediatric patients. Taylor & Francis 2020-06-09 /pmc/articles/PMC7450259/ /pubmed/32419639 http://dx.doi.org/10.1080/22221751.2020.1771219 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Yuan, Chunhui
Zhu, Hongmin
Yang, Yuan
Cai, Xiaonan
Xiang, Feiyan
Wu, Huan
Yao, Cong
Xiang, Yun
Xiao, Han
Viral loads in throat and anal swabs in children infected with SARS-CoV-2
title Viral loads in throat and anal swabs in children infected with SARS-CoV-2
title_full Viral loads in throat and anal swabs in children infected with SARS-CoV-2
title_fullStr Viral loads in throat and anal swabs in children infected with SARS-CoV-2
title_full_unstemmed Viral loads in throat and anal swabs in children infected with SARS-CoV-2
title_short Viral loads in throat and anal swabs in children infected with SARS-CoV-2
title_sort viral loads in throat and anal swabs in children infected with sars-cov-2
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450259/
https://www.ncbi.nlm.nih.gov/pubmed/32419639
http://dx.doi.org/10.1080/22221751.2020.1771219
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