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MicroRNA-874-3p Aggravates Doxorubicin-Induced Renal Podocyte Injury via Targeting Methionine Sulfoxide Reductase B3

Clinical application of doxorubicin (Dox) is limited due to its serious side effects including nephrotoxicity, and kidney podocytes play important roles in renal diseases. MicroRNAs (miRNAs) are critical regulators associated with human diseases. The purpose of this study was to explore a novel targ...

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Autores principales: Dai, Yan, Gao, Meng, Li, Linxia, Mao, Zhang, Xu, Lina, Yin, Lianhong, Qi, Yan, Peng, Jinyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450315/
https://www.ncbi.nlm.nih.gov/pubmed/32908641
http://dx.doi.org/10.1155/2020/9481841
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author Dai, Yan
Gao, Meng
Li, Linxia
Mao, Zhang
Xu, Lina
Yin, Lianhong
Qi, Yan
Peng, Jinyong
author_facet Dai, Yan
Gao, Meng
Li, Linxia
Mao, Zhang
Xu, Lina
Yin, Lianhong
Qi, Yan
Peng, Jinyong
author_sort Dai, Yan
collection PubMed
description Clinical application of doxorubicin (Dox) is limited due to its serious side effects including nephrotoxicity, and kidney podocytes play important roles in renal diseases. MicroRNAs (miRNAs) are critical regulators associated with human diseases. The purpose of this study was to explore a novel target in adjusting Dox-induced renal podocyte injury. Through a double luciferase reporter gene experiment, it was found that miR-874-3p directly targeted methionine sulfoxide reductase B3 (MsrB3). During the tests of miR-874-3p inhibitor and MsrB3 siRNA in human podocytes or miR-874-3p antagomir in mice, we found that the expression levels of downstream oxidative stress and apoptosis-related proteins were regulated by miR-874-3p/MsrB3 signal to alleviate or aggravate renal podocyte injury. The data in the present work showed that miR-874-3p aggravated Dox-caused renal podocyte injury by promoting apoptosis and oxidative damage via inhibiting MsrB3. Therefore, miR-874-3p/MsrB3 should be considered as a new therapeutic target in controlling renal podocyte injury induced by Dox.
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spelling pubmed-74503152020-09-08 MicroRNA-874-3p Aggravates Doxorubicin-Induced Renal Podocyte Injury via Targeting Methionine Sulfoxide Reductase B3 Dai, Yan Gao, Meng Li, Linxia Mao, Zhang Xu, Lina Yin, Lianhong Qi, Yan Peng, Jinyong Oxid Med Cell Longev Research Article Clinical application of doxorubicin (Dox) is limited due to its serious side effects including nephrotoxicity, and kidney podocytes play important roles in renal diseases. MicroRNAs (miRNAs) are critical regulators associated with human diseases. The purpose of this study was to explore a novel target in adjusting Dox-induced renal podocyte injury. Through a double luciferase reporter gene experiment, it was found that miR-874-3p directly targeted methionine sulfoxide reductase B3 (MsrB3). During the tests of miR-874-3p inhibitor and MsrB3 siRNA in human podocytes or miR-874-3p antagomir in mice, we found that the expression levels of downstream oxidative stress and apoptosis-related proteins were regulated by miR-874-3p/MsrB3 signal to alleviate or aggravate renal podocyte injury. The data in the present work showed that miR-874-3p aggravated Dox-caused renal podocyte injury by promoting apoptosis and oxidative damage via inhibiting MsrB3. Therefore, miR-874-3p/MsrB3 should be considered as a new therapeutic target in controlling renal podocyte injury induced by Dox. Hindawi 2020-08-18 /pmc/articles/PMC7450315/ /pubmed/32908641 http://dx.doi.org/10.1155/2020/9481841 Text en Copyright © 2020 Yan Dai et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dai, Yan
Gao, Meng
Li, Linxia
Mao, Zhang
Xu, Lina
Yin, Lianhong
Qi, Yan
Peng, Jinyong
MicroRNA-874-3p Aggravates Doxorubicin-Induced Renal Podocyte Injury via Targeting Methionine Sulfoxide Reductase B3
title MicroRNA-874-3p Aggravates Doxorubicin-Induced Renal Podocyte Injury via Targeting Methionine Sulfoxide Reductase B3
title_full MicroRNA-874-3p Aggravates Doxorubicin-Induced Renal Podocyte Injury via Targeting Methionine Sulfoxide Reductase B3
title_fullStr MicroRNA-874-3p Aggravates Doxorubicin-Induced Renal Podocyte Injury via Targeting Methionine Sulfoxide Reductase B3
title_full_unstemmed MicroRNA-874-3p Aggravates Doxorubicin-Induced Renal Podocyte Injury via Targeting Methionine Sulfoxide Reductase B3
title_short MicroRNA-874-3p Aggravates Doxorubicin-Induced Renal Podocyte Injury via Targeting Methionine Sulfoxide Reductase B3
title_sort microrna-874-3p aggravates doxorubicin-induced renal podocyte injury via targeting methionine sulfoxide reductase b3
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450315/
https://www.ncbi.nlm.nih.gov/pubmed/32908641
http://dx.doi.org/10.1155/2020/9481841
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