Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications

[Image: see text] Gelatin-based water-insoluble nanofibers with a diameter of 160 nm were obtained from electrospinning aqueous solutions containing gelatin with phenolic hydroxyl (Ph) moieties (Gelatin-Ph) and horseradish peroxidase (HRP). The water insolubility of the nanofibers was accomplished t...

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Autores principales: Furuno, Kotoko, Wang, Jing, Suzuki, Keiichiro, Nakahata, Masaki, Sakai, Shinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450611/
https://www.ncbi.nlm.nih.gov/pubmed/32875262
http://dx.doi.org/10.1021/acsomega.0c03164
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author Furuno, Kotoko
Wang, Jing
Suzuki, Keiichiro
Nakahata, Masaki
Sakai, Shinji
author_facet Furuno, Kotoko
Wang, Jing
Suzuki, Keiichiro
Nakahata, Masaki
Sakai, Shinji
author_sort Furuno, Kotoko
collection PubMed
description [Image: see text] Gelatin-based water-insoluble nanofibers with a diameter of 160 nm were obtained from electrospinning aqueous solutions containing gelatin with phenolic hydroxyl (Ph) moieties (Gelatin-Ph) and horseradish peroxidase (HRP). The water insolubility of the nanofibers was accomplished through HRP-catalyzed cross-linking of the Ph moieties by exposing the electrospun nanofibers to air containing hydrogen peroxide. The HRP activity in the electrospun nanofibers was 65% that of native HRP. The cytocompatibility necessary for tissue engineering applications of the water-insoluble Gelatin-Ph nanofibers was confirmed by the adhesion and viability of human embryonic kidney-derived HEK293 cells.
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spelling pubmed-74506112020-08-31 Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications Furuno, Kotoko Wang, Jing Suzuki, Keiichiro Nakahata, Masaki Sakai, Shinji ACS Omega [Image: see text] Gelatin-based water-insoluble nanofibers with a diameter of 160 nm were obtained from electrospinning aqueous solutions containing gelatin with phenolic hydroxyl (Ph) moieties (Gelatin-Ph) and horseradish peroxidase (HRP). The water insolubility of the nanofibers was accomplished through HRP-catalyzed cross-linking of the Ph moieties by exposing the electrospun nanofibers to air containing hydrogen peroxide. The HRP activity in the electrospun nanofibers was 65% that of native HRP. The cytocompatibility necessary for tissue engineering applications of the water-insoluble Gelatin-Ph nanofibers was confirmed by the adhesion and viability of human embryonic kidney-derived HEK293 cells. American Chemical Society 2020-08-13 /pmc/articles/PMC7450611/ /pubmed/32875262 http://dx.doi.org/10.1021/acsomega.0c03164 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Furuno, Kotoko
Wang, Jing
Suzuki, Keiichiro
Nakahata, Masaki
Sakai, Shinji
Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications
title Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications
title_full Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications
title_fullStr Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications
title_full_unstemmed Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications
title_short Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications
title_sort gelatin-based electrospun fibers insolubilized by horseradish peroxidase-catalyzed cross-linking for biomedical applications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450611/
https://www.ncbi.nlm.nih.gov/pubmed/32875262
http://dx.doi.org/10.1021/acsomega.0c03164
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