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Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena
BACKGROUND: Wheat blast, caused by Magnaporthe oryzae Triticum (MoT) pathotype, is a global threat to wheat (Triticum aestivum L.) production. Few blast resistance (R) genes have been identified to date, therefore assessing potential sources of resistance in wheat is important. The Brazilian wheat c...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7451118/ https://www.ncbi.nlm.nih.gov/pubmed/32854622 http://dx.doi.org/10.1186/s12870-020-02592-0 |
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author | Goddard, Rachel Steed, Andrew Chinoy, Catherine Ferreira, Jéssica Rosset Scheeren, Pedro Luiz Maciel, João Leodato Nunes Caierão, Eduardo Torres, Gisele Abigail Montan Consoli, Luciano Santana, Flavio Martins Fernandes, José Mauricio Cunha Simmonds, James Uauy, Cristobal Cockram, James Nicholson, Paul |
author_facet | Goddard, Rachel Steed, Andrew Chinoy, Catherine Ferreira, Jéssica Rosset Scheeren, Pedro Luiz Maciel, João Leodato Nunes Caierão, Eduardo Torres, Gisele Abigail Montan Consoli, Luciano Santana, Flavio Martins Fernandes, José Mauricio Cunha Simmonds, James Uauy, Cristobal Cockram, James Nicholson, Paul |
author_sort | Goddard, Rachel |
collection | PubMed |
description | BACKGROUND: Wheat blast, caused by Magnaporthe oryzae Triticum (MoT) pathotype, is a global threat to wheat (Triticum aestivum L.) production. Few blast resistance (R) genes have been identified to date, therefore assessing potential sources of resistance in wheat is important. The Brazilian wheat cultivar BR 18-Terena is considered one of the best sources of resistance to blast and has been widely used in Brazilian breeding programmes, however the underlying genetics of this resistance are unknown. RESULTS: BR 18-Terena was used as the common parent in the development of two recombinant inbred line (RIL) F(6) populations with the Brazilian cultivars Anahuac 75 and BRS 179. Populations were phenotyped for resistance at the seedling and heading stage using the sequenced MoT isolate BR32, with transgressive segregation being observed. Genetic maps containing 1779 and 1318 markers, were produced for the Anahuac 75 × BR 18-Terena and BR 18-Terena × BRS 179 populations, respectively. Five quantitative trait loci (QTL) associated with seedling resistance, on chromosomes 2B, 4B (2 QTL), 5A and 6A, were identified, as were four QTL associated with heading stage resistance (1A, 2B, 4A and 5A). Seedling and heading stage QTL did not co-locate, despite a significant positive correlation between these traits, indicating that resistance at these developmental stages is likely to be controlled by different genes. BR 18-Terena provided the resistant allele for six QTL, at both developmental stages, with the largest phenotypic effect conferred by a QTL being 24.8% suggesting that BR 18-Terena possesses quantitative resistance. Haplotype analysis of 100 Brazilian wheat cultivars indicates that 11.0% of cultivars already possess a BR 18-Terena-like haplotype for more than one of the identified heading stage QTL. CONCLUSIONS: This study suggests that BR 18-Terena possesses quantitative resistance to wheat blast, with nine QTL associated with resistance at either the seedling or heading stage being detected. Wheat blast resistance is also largely tissue-specific. Identification of durable quantitative resistances which can be combined with race-specific R gene-mediated resistance is critical to effectively control wheat blast. Collectively, this work facilitates marker-assisted selection to develop new varieties for cultivation in regions at risk from this emerging disease. |
format | Online Article Text |
id | pubmed-7451118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-74511182020-08-28 Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena Goddard, Rachel Steed, Andrew Chinoy, Catherine Ferreira, Jéssica Rosset Scheeren, Pedro Luiz Maciel, João Leodato Nunes Caierão, Eduardo Torres, Gisele Abigail Montan Consoli, Luciano Santana, Flavio Martins Fernandes, José Mauricio Cunha Simmonds, James Uauy, Cristobal Cockram, James Nicholson, Paul BMC Plant Biol Research Article BACKGROUND: Wheat blast, caused by Magnaporthe oryzae Triticum (MoT) pathotype, is a global threat to wheat (Triticum aestivum L.) production. Few blast resistance (R) genes have been identified to date, therefore assessing potential sources of resistance in wheat is important. The Brazilian wheat cultivar BR 18-Terena is considered one of the best sources of resistance to blast and has been widely used in Brazilian breeding programmes, however the underlying genetics of this resistance are unknown. RESULTS: BR 18-Terena was used as the common parent in the development of two recombinant inbred line (RIL) F(6) populations with the Brazilian cultivars Anahuac 75 and BRS 179. Populations were phenotyped for resistance at the seedling and heading stage using the sequenced MoT isolate BR32, with transgressive segregation being observed. Genetic maps containing 1779 and 1318 markers, were produced for the Anahuac 75 × BR 18-Terena and BR 18-Terena × BRS 179 populations, respectively. Five quantitative trait loci (QTL) associated with seedling resistance, on chromosomes 2B, 4B (2 QTL), 5A and 6A, were identified, as were four QTL associated with heading stage resistance (1A, 2B, 4A and 5A). Seedling and heading stage QTL did not co-locate, despite a significant positive correlation between these traits, indicating that resistance at these developmental stages is likely to be controlled by different genes. BR 18-Terena provided the resistant allele for six QTL, at both developmental stages, with the largest phenotypic effect conferred by a QTL being 24.8% suggesting that BR 18-Terena possesses quantitative resistance. Haplotype analysis of 100 Brazilian wheat cultivars indicates that 11.0% of cultivars already possess a BR 18-Terena-like haplotype for more than one of the identified heading stage QTL. CONCLUSIONS: This study suggests that BR 18-Terena possesses quantitative resistance to wheat blast, with nine QTL associated with resistance at either the seedling or heading stage being detected. Wheat blast resistance is also largely tissue-specific. Identification of durable quantitative resistances which can be combined with race-specific R gene-mediated resistance is critical to effectively control wheat blast. Collectively, this work facilitates marker-assisted selection to develop new varieties for cultivation in regions at risk from this emerging disease. BioMed Central 2020-08-27 /pmc/articles/PMC7451118/ /pubmed/32854622 http://dx.doi.org/10.1186/s12870-020-02592-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Goddard, Rachel Steed, Andrew Chinoy, Catherine Ferreira, Jéssica Rosset Scheeren, Pedro Luiz Maciel, João Leodato Nunes Caierão, Eduardo Torres, Gisele Abigail Montan Consoli, Luciano Santana, Flavio Martins Fernandes, José Mauricio Cunha Simmonds, James Uauy, Cristobal Cockram, James Nicholson, Paul Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena |
title | Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena |
title_full | Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena |
title_fullStr | Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena |
title_full_unstemmed | Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena |
title_short | Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena |
title_sort | dissecting the genetic basis of wheat blast resistance in the brazilian wheat cultivar br 18-terena |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7451118/ https://www.ncbi.nlm.nih.gov/pubmed/32854622 http://dx.doi.org/10.1186/s12870-020-02592-0 |
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