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Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment
Distinct lineages of Gammaproteobacteria clade Woeseiales are globally distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing chang...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7451513/ https://www.ncbi.nlm.nih.gov/pubmed/32853201 http://dx.doi.org/10.1371/journal.pone.0234839 |
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author | Buongiorno, Joy Sipes, Katie Wasmund, Kenneth Loy, Alexander Lloyd, Karen G. |
author_facet | Buongiorno, Joy Sipes, Katie Wasmund, Kenneth Loy, Alexander Lloyd, Karen G. |
author_sort | Buongiorno, Joy |
collection | PubMed |
description | Distinct lineages of Gammaproteobacteria clade Woeseiales are globally distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing change. This study combined 16S rRNA gene analysis, metagenome-assembled genomes (MAGs), and genome-resolved metatranscriptomics uncovered the in situ abundance and transcriptional activity of Woeseiales with burial in four shallow sediment sites of Kongsfjorden and Van Keulenfjorden of Svalbard (79°N). We present five novel Woeseiales MAGs and show transcriptional evidence for metabolic plasticity during burial, including sulfur oxidation with reverse dissimilatory sulfite reductase (dsrAB) down to 4 cm depth and nitrite reduction down to 6 cm depth. A single stress protein, spore protein SP21 (hspA), had a tenfold higher mRNA abundance than any other transcript, and was a hundredfold higher on average than other transcripts. At three out of the four sites, SP21 transcript abundance increased with depth, while total mRNA abundance and richness decreased, indicating a shift in investment from metabolism and other cellular processes to build-up of spore protein SP21. The SP21 gene in MAGs was often flanked by genes involved in membrane-associated stress response. The ability of Woeseiales to shift from sulfur oxidation to nitrite reduction with burial into marine sediments with decreasing access to overlying oxic bottom waters, as well as enter into a dormant state dominated by SP21, may account for its ubiquity and high abundance in marine sediments worldwide, including those of the rapidly shifting Arctic. |
format | Online Article Text |
id | pubmed-7451513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-74515132020-09-02 Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment Buongiorno, Joy Sipes, Katie Wasmund, Kenneth Loy, Alexander Lloyd, Karen G. PLoS One Research Article Distinct lineages of Gammaproteobacteria clade Woeseiales are globally distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing change. This study combined 16S rRNA gene analysis, metagenome-assembled genomes (MAGs), and genome-resolved metatranscriptomics uncovered the in situ abundance and transcriptional activity of Woeseiales with burial in four shallow sediment sites of Kongsfjorden and Van Keulenfjorden of Svalbard (79°N). We present five novel Woeseiales MAGs and show transcriptional evidence for metabolic plasticity during burial, including sulfur oxidation with reverse dissimilatory sulfite reductase (dsrAB) down to 4 cm depth and nitrite reduction down to 6 cm depth. A single stress protein, spore protein SP21 (hspA), had a tenfold higher mRNA abundance than any other transcript, and was a hundredfold higher on average than other transcripts. At three out of the four sites, SP21 transcript abundance increased with depth, while total mRNA abundance and richness decreased, indicating a shift in investment from metabolism and other cellular processes to build-up of spore protein SP21. The SP21 gene in MAGs was often flanked by genes involved in membrane-associated stress response. The ability of Woeseiales to shift from sulfur oxidation to nitrite reduction with burial into marine sediments with decreasing access to overlying oxic bottom waters, as well as enter into a dormant state dominated by SP21, may account for its ubiquity and high abundance in marine sediments worldwide, including those of the rapidly shifting Arctic. Public Library of Science 2020-08-27 /pmc/articles/PMC7451513/ /pubmed/32853201 http://dx.doi.org/10.1371/journal.pone.0234839 Text en © 2020 Buongiorno et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Buongiorno, Joy Sipes, Katie Wasmund, Kenneth Loy, Alexander Lloyd, Karen G. Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title | Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title_full | Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title_fullStr | Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title_full_unstemmed | Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title_short | Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title_sort | woeseiales transcriptional response to shallow burial in arctic fjord surface sediment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7451513/ https://www.ncbi.nlm.nih.gov/pubmed/32853201 http://dx.doi.org/10.1371/journal.pone.0234839 |
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