Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19

The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencin...

Descripción completa

Detalles Bibliográficos
Autores principales: Hou, Tieying, Zeng, Weiqi, Yang, Minling, Chen, Wenjing, Ren, Lili, Ai, Jingwen, Wu, Ji, Liao, Yalong, Gou, Xuejing, Li, Yongjun, Wang, Xiaorui, Su, Hang, Gu, Bing, Wang, Jianwei, Xu, Teng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7451577/
https://www.ncbi.nlm.nih.gov/pubmed/32853291
http://dx.doi.org/10.1371/journal.ppat.1008705
Descripción
Sumario:The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic coronavirus has raised great public health concern globally. Rapid and accurate diagnosis of this novel pathogen posts great challenges not only clinically but also technologically. Metagenomic next-generation sequencing (mNGS) and reverse-transcription PCR (RT-PCR) have been the most commonly used molecular methodologies. However, each has their own limitations. In this study, we developed an isothermal, CRISPR-based diagnostic for COVID-19 with near single-copy sensitivity. The diagnostic performances of all three technology platforms were also compared. Our study aimed to provide more insights into the molecular detection of SARS-CoV-2, and also to present a novel diagnostic option for this new emerging virus.

Ejemplares similares