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The Chromosome-Scale Genome of Melon Dissects Genetic Architecture of Important Agronomic Traits
Comparative and evolutionary genomics analyses are the powerful tools to provide mechanistic insights into important agronomic traits. Here, we completed a chromosome-scale assembly of the “neglected” but vital melon subspecies Cucumis melo ssp. agrestis using single-molecule real-time sequencing, H...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7452659/ https://www.ncbi.nlm.nih.gov/pubmed/32798971 http://dx.doi.org/10.1016/j.isci.2020.101422 |
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author | Yang, Jinghua Deng, Guancong Lian, Jinmin Garraway, Jenella Niu, Yongchao Hu, Zhongyuan Yu, Jingquan Zhang, Mingfang |
author_facet | Yang, Jinghua Deng, Guancong Lian, Jinmin Garraway, Jenella Niu, Yongchao Hu, Zhongyuan Yu, Jingquan Zhang, Mingfang |
author_sort | Yang, Jinghua |
collection | PubMed |
description | Comparative and evolutionary genomics analyses are the powerful tools to provide mechanistic insights into important agronomic traits. Here, we completed a chromosome-scale assembly of the “neglected” but vital melon subspecies Cucumis melo ssp. agrestis using single-molecule real-time sequencing, Hi-C, and an ultra-dense genetic map. Comparative genomics analyses identified two targeted genes, UDP-sugar pyrophosphorylase and α-galactosidase, that were selected during evolution for specific phloem transport of oligosaccharides in Cucurbitaceae. Association analysis of transcriptome and the DNA methylation patterns revealed the epigenetic regulation of sucrose accumulation in developing fruits. We constructed the melon recombinant inbred lines to uncover Alkaline/Neutral Invertase (CINV), Sucrose-Phosphatase 2 (SPP2), α-galactosidase, and β-galactosidase loci related to sucrose accumulation and an LRR receptor-like serine/threonine-protein kinase associated with gummy stem blight resistance. This study provides essential genomic resources enabling functional genomics studies and the genomics-informed breeding pipelines for improving the fruit quality and disease resistance traits. |
format | Online Article Text |
id | pubmed-7452659 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-74526592020-09-03 The Chromosome-Scale Genome of Melon Dissects Genetic Architecture of Important Agronomic Traits Yang, Jinghua Deng, Guancong Lian, Jinmin Garraway, Jenella Niu, Yongchao Hu, Zhongyuan Yu, Jingquan Zhang, Mingfang iScience Article Comparative and evolutionary genomics analyses are the powerful tools to provide mechanistic insights into important agronomic traits. Here, we completed a chromosome-scale assembly of the “neglected” but vital melon subspecies Cucumis melo ssp. agrestis using single-molecule real-time sequencing, Hi-C, and an ultra-dense genetic map. Comparative genomics analyses identified two targeted genes, UDP-sugar pyrophosphorylase and α-galactosidase, that were selected during evolution for specific phloem transport of oligosaccharides in Cucurbitaceae. Association analysis of transcriptome and the DNA methylation patterns revealed the epigenetic regulation of sucrose accumulation in developing fruits. We constructed the melon recombinant inbred lines to uncover Alkaline/Neutral Invertase (CINV), Sucrose-Phosphatase 2 (SPP2), α-galactosidase, and β-galactosidase loci related to sucrose accumulation and an LRR receptor-like serine/threonine-protein kinase associated with gummy stem blight resistance. This study provides essential genomic resources enabling functional genomics studies and the genomics-informed breeding pipelines for improving the fruit quality and disease resistance traits. Elsevier 2020-07-29 /pmc/articles/PMC7452659/ /pubmed/32798971 http://dx.doi.org/10.1016/j.isci.2020.101422 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Yang, Jinghua Deng, Guancong Lian, Jinmin Garraway, Jenella Niu, Yongchao Hu, Zhongyuan Yu, Jingquan Zhang, Mingfang The Chromosome-Scale Genome of Melon Dissects Genetic Architecture of Important Agronomic Traits |
title | The Chromosome-Scale Genome of Melon Dissects Genetic Architecture of Important Agronomic Traits |
title_full | The Chromosome-Scale Genome of Melon Dissects Genetic Architecture of Important Agronomic Traits |
title_fullStr | The Chromosome-Scale Genome of Melon Dissects Genetic Architecture of Important Agronomic Traits |
title_full_unstemmed | The Chromosome-Scale Genome of Melon Dissects Genetic Architecture of Important Agronomic Traits |
title_short | The Chromosome-Scale Genome of Melon Dissects Genetic Architecture of Important Agronomic Traits |
title_sort | chromosome-scale genome of melon dissects genetic architecture of important agronomic traits |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7452659/ https://www.ncbi.nlm.nih.gov/pubmed/32798971 http://dx.doi.org/10.1016/j.isci.2020.101422 |
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