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Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation

BACKGROUND: Human bone marrow stromal/stem cells (hMSCs, also known as the skeletal stem cells or mesenchymal stem cells) are being employed to study lineage fate determination to osteoblasts, adipocytes, and chondrocytes. However, mechanistic studies employing hMSC have been hampered by the difficu...

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Detalles Bibliográficos
Autores principales:	Chen, Li, Shi, Kaikai, Qiu, Weimin, Aagaard, Lars, Kassem, Moustapha
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7453244/ https://www.ncbi.nlm.nih.gov/pubmed/32922451 http://dx.doi.org/10.1155/2020/8857344

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