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Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice
Application of green fluorescent protein (GFP) in a variety of biosystems as a unique bioindicator or biomarker has revolutionized biological research and made groundbreaking achievements, while increasing evidence has shown alterations in biological properties and physiological functions of the cel...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7453463/ https://www.ncbi.nlm.nih.gov/pubmed/32922229 http://dx.doi.org/10.1177/1559325820951332 |
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author | Liu, Cuihua Tanaka, Kaoru Katsube, Takanori Varès, Guillaume Maruyama, Kouichi Ninomiya, Yasuharu Fardous, Zeenath Sun, Chao Fujimori, Akira Moreno, Stéphanie G. Nenoi, Mitsuru Wang, Bing |
author_facet | Liu, Cuihua Tanaka, Kaoru Katsube, Takanori Varès, Guillaume Maruyama, Kouichi Ninomiya, Yasuharu Fardous, Zeenath Sun, Chao Fujimori, Akira Moreno, Stéphanie G. Nenoi, Mitsuru Wang, Bing |
author_sort | Liu, Cuihua |
collection | PubMed |
description | Application of green fluorescent protein (GFP) in a variety of biosystems as a unique bioindicator or biomarker has revolutionized biological research and made groundbreaking achievements, while increasing evidence has shown alterations in biological properties and physiological functions of the cells and animals overexpressing transgenic GFP. In this work, response to total body irradiation (TBI) was comparatively studied in GFP transgenic C57BL/6-Tg (CAG-EGFP) mice and C57BL/6 N wild type mice. It was demonstrated that GFP transgenic mice were more sensitive to radiation-induced bone marrow death, and no adaptive response could be induced. In the nucleated bone marrow cells of GFP transgenic mice exposed to a middle dose, there was a significant increase in both the percentage of cells expressing pro-apoptotic gene Bax and apoptotic cell death. While in wild type cells, lower expression of pro-apoptotic gene Bax and higher expression of anti-apoptotic gene Bcl-2, and significant lower induction of apoptosis were observed compared to GFP transgenic cells. Results suggest that presence of GFP could alter response to TBI at whole body, cellular and molecular levels in mice. These findings indicate that there could be a major influence on the interpretation of the results obtained in GFP transgenic mice. |
format | Online Article Text |
id | pubmed-7453463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-74534632020-09-11 Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice Liu, Cuihua Tanaka, Kaoru Katsube, Takanori Varès, Guillaume Maruyama, Kouichi Ninomiya, Yasuharu Fardous, Zeenath Sun, Chao Fujimori, Akira Moreno, Stéphanie G. Nenoi, Mitsuru Wang, Bing Dose Response Potential Biomarkers of Radiation Damage Application of green fluorescent protein (GFP) in a variety of biosystems as a unique bioindicator or biomarker has revolutionized biological research and made groundbreaking achievements, while increasing evidence has shown alterations in biological properties and physiological functions of the cells and animals overexpressing transgenic GFP. In this work, response to total body irradiation (TBI) was comparatively studied in GFP transgenic C57BL/6-Tg (CAG-EGFP) mice and C57BL/6 N wild type mice. It was demonstrated that GFP transgenic mice were more sensitive to radiation-induced bone marrow death, and no adaptive response could be induced. In the nucleated bone marrow cells of GFP transgenic mice exposed to a middle dose, there was a significant increase in both the percentage of cells expressing pro-apoptotic gene Bax and apoptotic cell death. While in wild type cells, lower expression of pro-apoptotic gene Bax and higher expression of anti-apoptotic gene Bcl-2, and significant lower induction of apoptosis were observed compared to GFP transgenic cells. Results suggest that presence of GFP could alter response to TBI at whole body, cellular and molecular levels in mice. These findings indicate that there could be a major influence on the interpretation of the results obtained in GFP transgenic mice. SAGE Publications 2020-08-26 /pmc/articles/PMC7453463/ /pubmed/32922229 http://dx.doi.org/10.1177/1559325820951332 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Potential Biomarkers of Radiation Damage Liu, Cuihua Tanaka, Kaoru Katsube, Takanori Varès, Guillaume Maruyama, Kouichi Ninomiya, Yasuharu Fardous, Zeenath Sun, Chao Fujimori, Akira Moreno, Stéphanie G. Nenoi, Mitsuru Wang, Bing Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice |
title | Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice |
title_full | Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice |
title_fullStr | Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice |
title_full_unstemmed | Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice |
title_short | Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice |
title_sort | altered response to total body irradiation of c57bl/6-tg (cag-egfp) mice |
topic | Potential Biomarkers of Radiation Damage |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7453463/ https://www.ncbi.nlm.nih.gov/pubmed/32922229 http://dx.doi.org/10.1177/1559325820951332 |
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