Electroacupuncture pre-conditioning protects from lung injury induced by limb ischemia/reperfusion through TLR4 and NF-κB in rats

Limb ischemia/reperfusion (I/R) can induce inflammation, causing acute lung injury. The Toll-like receptor 4 (TLR4)/NF-κB pathway plays an important role in acute and chronic inflammatory disorders. Several studies have demonstrated the efficacy of acupuncture in lung inflammatory injury. The aim of the present study was to elucidate the mechanism underlying the protective effect of electroacupuncture (EA) against lung injury induced by limb I/R. EA applied at the Zusanli and Sanyinjiao acupoints attenuated lung injury and decreased the secretion of inflammatory factors such as tumor necrosis factor-α, interleukin (IL)-1, IL-6 and myeloperoxidase. Moreover, the expression levels of TLR4 and NF-κB were suppressed by EA. Thus, the present findings suggested that EA can reduce pulmonary inflammation induced by limb I/R injury, possibly via the inhibition of the TLR4/NF-κB pathway.