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Expression Pattern of Early Growth Response Gene 1 during Olive Flounder (Paralichthys olivaceus) Embryonic Development

The early growth response protein 1 (Egr-1) is a widely reported zinc finger protein and a well known transcription factor encoded by the Egr-1 gene, which plays key roles in many aspects of vertebrate embryogenesis and in adult vertebrates. The Egr-1 expression is important in the formation of the...

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Autores principales: Yang, Hyun, Lee, Jeong-Ho, Noh, Jae Koo, Kim, Hyun Chul, Park, Choul-Ji, Park, Jong-Won, Kim, Kyung-Kil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Developmental Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455093/
https://www.ncbi.nlm.nih.gov/pubmed/32885106
http://dx.doi.org/10.12717/DR.2014.18.4.233
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author Yang, Hyun
Lee, Jeong-Ho
Noh, Jae Koo
Kim, Hyun Chul
Park, Choul-Ji
Park, Jong-Won
Kim, Kyung-Kil
author_facet Yang, Hyun
Lee, Jeong-Ho
Noh, Jae Koo
Kim, Hyun Chul
Park, Choul-Ji
Park, Jong-Won
Kim, Kyung-Kil
author_sort Yang, Hyun
collection PubMed
description The early growth response protein 1 (Egr-1) is a widely reported zinc finger protein and a well known transcription factor encoded by the Egr-1 gene, which plays key roles in many aspects of vertebrate embryogenesis and in adult vertebrates. The Egr-1 expression is important in the formation of the gill vascular system in flounders, which develops during the post-hatching phase and is essential for survival during the juvenile period. However, the complete details of Egr-1 expression during embryo development in olive flounder are not available. We assessed the expression patterns of Egr-1 during the early development of olive flounders by using reverse transcription polymerase chain reaction (RT-PCR) analysis. Microscopic observations showed that gill filament formation corresponded with the Egr-1 expression. Thus, we showed that Egr-1 plays a vital role in angiogenesis in the gill filaments during embryogenesis. Further, Egr-1 expression was found to be strong at 5 days after hatching (DAH), in the development of the gill vascular system, and this strong expression level was maintained throughout all the development stages. Our findings have important implications with respect to the biological role of Egr-1 and evolution of the first respiratory blood vessels in the gills of olive flounder. Further studies are required to elucidate the Egr-1-mediated stress response and to decipher the functional role of Egr-1 in developmental stages.
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spelling pubmed-74550932020-09-02 Expression Pattern of Early Growth Response Gene 1 during Olive Flounder (Paralichthys olivaceus) Embryonic Development Yang, Hyun Lee, Jeong-Ho Noh, Jae Koo Kim, Hyun Chul Park, Choul-Ji Park, Jong-Won Kim, Kyung-Kil Dev Reprod Article The early growth response protein 1 (Egr-1) is a widely reported zinc finger protein and a well known transcription factor encoded by the Egr-1 gene, which plays key roles in many aspects of vertebrate embryogenesis and in adult vertebrates. The Egr-1 expression is important in the formation of the gill vascular system in flounders, which develops during the post-hatching phase and is essential for survival during the juvenile period. However, the complete details of Egr-1 expression during embryo development in olive flounder are not available. We assessed the expression patterns of Egr-1 during the early development of olive flounders by using reverse transcription polymerase chain reaction (RT-PCR) analysis. Microscopic observations showed that gill filament formation corresponded with the Egr-1 expression. Thus, we showed that Egr-1 plays a vital role in angiogenesis in the gill filaments during embryogenesis. Further, Egr-1 expression was found to be strong at 5 days after hatching (DAH), in the development of the gill vascular system, and this strong expression level was maintained throughout all the development stages. Our findings have important implications with respect to the biological role of Egr-1 and evolution of the first respiratory blood vessels in the gills of olive flounder. Further studies are required to elucidate the Egr-1-mediated stress response and to decipher the functional role of Egr-1 in developmental stages. Korean Society of Developmental Biology 2014-12 /pmc/articles/PMC7455093/ /pubmed/32885106 http://dx.doi.org/10.12717/DR.2014.18.4.233 Text en © Copyright an Official Journal of the Korean Society of Developmental Biology. All Rights Reserve http://creativecommons.org/licenses/by-nc/3.0/ This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Yang, Hyun
Lee, Jeong-Ho
Noh, Jae Koo
Kim, Hyun Chul
Park, Choul-Ji
Park, Jong-Won
Kim, Kyung-Kil
Expression Pattern of Early Growth Response Gene 1 during Olive Flounder (Paralichthys olivaceus) Embryonic Development
title Expression Pattern of Early Growth Response Gene 1 during Olive Flounder (Paralichthys olivaceus) Embryonic Development
title_full Expression Pattern of Early Growth Response Gene 1 during Olive Flounder (Paralichthys olivaceus) Embryonic Development
title_fullStr Expression Pattern of Early Growth Response Gene 1 during Olive Flounder (Paralichthys olivaceus) Embryonic Development
title_full_unstemmed Expression Pattern of Early Growth Response Gene 1 during Olive Flounder (Paralichthys olivaceus) Embryonic Development
title_short Expression Pattern of Early Growth Response Gene 1 during Olive Flounder (Paralichthys olivaceus) Embryonic Development
title_sort expression pattern of early growth response gene 1 during olive flounder (paralichthys olivaceus) embryonic development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455093/
https://www.ncbi.nlm.nih.gov/pubmed/32885106
http://dx.doi.org/10.12717/DR.2014.18.4.233
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