A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos

In all animal species, oxygen consumption is a key process that is partially impaired in a large number of pathological situations and thus provides informative details on the physiopathology of the disease. In this study, we describe a simple and affordable method to precisely measure oxygen consum...

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Autores principales: Somkhit, Julie, Loyant, Roxane, Brenet, Alexandre, Hassan-Abdi, Rahma, Yanicostas, Constantin, Porceddu, Mathieu, Borgne-Sanchez, Annie, Soussi-Yanicostas, Nadia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2020
Materias:
TechnoFish—Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455472/
https://www.ncbi.nlm.nih.gov/pubmed/32364833
http://dx.doi.org/10.1089/zeb.2020.1878
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author Somkhit, Julie
Loyant, Roxane
Brenet, Alexandre
Hassan-Abdi, Rahma
Yanicostas, Constantin
Porceddu, Mathieu
Borgne-Sanchez, Annie
Soussi-Yanicostas, Nadia
author_facet Somkhit, Julie
Loyant, Roxane
Brenet, Alexandre
Hassan-Abdi, Rahma
Yanicostas, Constantin
Porceddu, Mathieu
Borgne-Sanchez, Annie
Soussi-Yanicostas, Nadia
author_sort Somkhit, Julie
collection PubMed
description In all animal species, oxygen consumption is a key process that is partially impaired in a large number of pathological situations and thus provides informative details on the physiopathology of the disease. In this study, we describe a simple and affordable method to precisely measure oxygen consumption in living zebrafish larvae using a spectrofluorometer and the MitoXpress Xtra Oxygen Consumption Assay. In addition, we used zebrafish larvae treated with mitochondrial respiratory chain inhibitors, antimycin A or rotenone, to verify that our method enables precise and reliable measurements of oxygen consumption.
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spelling pubmed-74554722020-08-31 A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos Somkhit, Julie Loyant, Roxane Brenet, Alexandre Hassan-Abdi, Rahma Yanicostas, Constantin Porceddu, Mathieu Borgne-Sanchez, Annie Soussi-Yanicostas, Nadia Zebrafish TechnoFish—Methods In all animal species, oxygen consumption is a key process that is partially impaired in a large number of pathological situations and thus provides informative details on the physiopathology of the disease. In this study, we describe a simple and affordable method to precisely measure oxygen consumption in living zebrafish larvae using a spectrofluorometer and the MitoXpress Xtra Oxygen Consumption Assay. In addition, we used zebrafish larvae treated with mitochondrial respiratory chain inhibitors, antimycin A or rotenone, to verify that our method enables precise and reliable measurements of oxygen consumption. Mary Ann Liebert, Inc., publishers 2020-08-01 2020-08-13 /pmc/articles/PMC7455472/ /pubmed/32364833 http://dx.doi.org/10.1089/zeb.2020.1878 Text en © Julie Somkhit et al. 2020; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.
spellingShingle TechnoFish—Methods
Somkhit, Julie
Loyant, Roxane
Brenet, Alexandre
Hassan-Abdi, Rahma
Yanicostas, Constantin
Porceddu, Mathieu
Borgne-Sanchez, Annie
Soussi-Yanicostas, Nadia
A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos
title A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos
title_full A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos
title_fullStr A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos
title_full_unstemmed A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos
title_short A Fast, Simple, and Affordable Technique to Measure Oxygen Consumption in Living Zebrafish Embryos
title_sort fast, simple, and affordable technique to measure oxygen consumption in living zebrafish embryos
topic TechnoFish—Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455472/
https://www.ncbi.nlm.nih.gov/pubmed/32364833
http://dx.doi.org/10.1089/zeb.2020.1878
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