The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway

Soybean protein‐derived peptides (SBP) are a rich source of various bioactive peptides with multiple health benefits. However, the prospective effects of SBP on human cells are still unclear. Therefore, this article investigated the effects of small molecular weight SBP on MG132‐induced apoptosis in...

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Detalles Bibliográficos
Autores principales: Yi, Guofu, Li, He, Li, You, Zhao, Fen, Ying, Zhiwei, Liu, Menglan, Zhang, Jian, Liu, Xinqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455986/
https://www.ncbi.nlm.nih.gov/pubmed/32884739
http://dx.doi.org/10.1002/fsn3.1776
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author Yi, Guofu
Li, He
Li, You
Zhao, Fen
Ying, Zhiwei
Liu, Menglan
Zhang, Jian
Liu, Xinqi
author_facet Yi, Guofu
Li, He
Li, You
Zhao, Fen
Ying, Zhiwei
Liu, Menglan
Zhang, Jian
Liu, Xinqi
author_sort Yi, Guofu
collection PubMed
description Soybean protein‐derived peptides (SBP) are a rich source of various bioactive peptides with multiple health benefits. However, the prospective effects of SBP on human cells are still unclear. Therefore, this article investigated the effects of small molecular weight SBP on MG132‐induced apoptosis in RAW264.7 cells. SBP inhibited MG132‐induced apoptosis of RAW264.7 cells in a dose‐dependent manner by flow cytometry. To further study its molecular mechanisms, Western blot analysis demonstrated that SBP could activate the PI3K‐AKT pathway by increasing the phosphorylation of PI3K and AKT and inhibiting apoptosis pathway by downregulating the expressions of pro‐apoptotic proteins of Bim, Bax, Fas, and Fasl and promoting the expressions of anti‐apoptotic proteins of Bcl‐xL and Bcl‐2. These results indicated the protective effect of SBP on MG132‐induced apoptosis in RAW264.7 cells.
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spelling pubmed-74559862020-09-02 The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway Yi, Guofu Li, He Li, You Zhao, Fen Ying, Zhiwei Liu, Menglan Zhang, Jian Liu, Xinqi Food Sci Nutr Original Research Soybean protein‐derived peptides (SBP) are a rich source of various bioactive peptides with multiple health benefits. However, the prospective effects of SBP on human cells are still unclear. Therefore, this article investigated the effects of small molecular weight SBP on MG132‐induced apoptosis in RAW264.7 cells. SBP inhibited MG132‐induced apoptosis of RAW264.7 cells in a dose‐dependent manner by flow cytometry. To further study its molecular mechanisms, Western blot analysis demonstrated that SBP could activate the PI3K‐AKT pathway by increasing the phosphorylation of PI3K and AKT and inhibiting apoptosis pathway by downregulating the expressions of pro‐apoptotic proteins of Bim, Bax, Fas, and Fasl and promoting the expressions of anti‐apoptotic proteins of Bcl‐xL and Bcl‐2. These results indicated the protective effect of SBP on MG132‐induced apoptosis in RAW264.7 cells. John Wiley and Sons Inc. 2020-07-16 /pmc/articles/PMC7455986/ /pubmed/32884739 http://dx.doi.org/10.1002/fsn3.1776 Text en © 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Yi, Guofu
Li, He
Li, You
Zhao, Fen
Ying, Zhiwei
Liu, Menglan
Zhang, Jian
Liu, Xinqi
The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway
title The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway
title_full The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway
title_fullStr The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway
title_full_unstemmed The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway
title_short The protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway
title_sort protective effect of soybean protein‐derived peptides on apoptosis via the activation of pi3k‐akt and inhibition on apoptosis pathway
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455986/
https://www.ncbi.nlm.nih.gov/pubmed/32884739
http://dx.doi.org/10.1002/fsn3.1776
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