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Comparison of three serological tests for the detection of Coxiella burnetii specific antibodies in European wild rabbits

BACKGROUND: Coxiella burnetii causes Q fever, a zoonotic bacterial disease with a multi-host cycle and reservoirs in wild and domestic animal species. Q fever has a significant impact on the Australian public health and economy but its ecology and contributing reservoir species remain poorly underst...

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Autores principales: Caraguel, Charles, Bassett, Sarah, González-Barrio, David, Elsworth, Peter, Chaber, Anne-Lise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456029/
https://www.ncbi.nlm.nih.gov/pubmed/32859195
http://dx.doi.org/10.1186/s12917-020-02526-w
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author Caraguel, Charles
Bassett, Sarah
González-Barrio, David
Elsworth, Peter
Chaber, Anne-Lise
author_facet Caraguel, Charles
Bassett, Sarah
González-Barrio, David
Elsworth, Peter
Chaber, Anne-Lise
author_sort Caraguel, Charles
collection PubMed
description BACKGROUND: Coxiella burnetii causes Q fever, a zoonotic bacterial disease with a multi-host cycle and reservoirs in wild and domestic animal species. Q fever has a significant impact on the Australian public health and economy but its ecology and contributing reservoir species remain poorly understood. In Europe, rabbits (Oryctolagus cuniculus) were identified as a major reservoir of C. burnetii and it is possible that they play a similar role in Australia. In absence of commercial kit available for rabbit, the Thermo Fisher - PrioCHECK™ Ruminant Q fever Ab Plate Kit was adapted to successfully screen rabbits population in Europe. However, this assay is not accessible in Australia and we assessed the equivalency of two commercially available kits in Australia – IDEXX - CHEKIT Q Fever Antibody ELISA kit and IDVet - ID Screen® Q Fever Indirect Multi-species with the Thermo Fisher kit (reference kit). RESULTS: A total of 94 rabbit sera were screened by all three ELISA kits using the same confirmed positive and negative controls. While the IDEXX kit failed to agree the other two assays (concordance correlation coefficient, r(b) < 0.77), IDVet kit showed satisfactory equivalency with Thermo Fisher (r(b) = 0.927). CONCLUSION: IDvet kit provides the best alternative for Thermo Fisher in the detection of C. burnetii specific antibodies in rabbits in Australia. Further trials are required to confirm these preliminary results due to the low seroprevalence of Coxiella burnetii observed in the study sera.
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spelling pubmed-74560292020-08-31 Comparison of three serological tests for the detection of Coxiella burnetii specific antibodies in European wild rabbits Caraguel, Charles Bassett, Sarah González-Barrio, David Elsworth, Peter Chaber, Anne-Lise BMC Vet Res Research Article BACKGROUND: Coxiella burnetii causes Q fever, a zoonotic bacterial disease with a multi-host cycle and reservoirs in wild and domestic animal species. Q fever has a significant impact on the Australian public health and economy but its ecology and contributing reservoir species remain poorly understood. In Europe, rabbits (Oryctolagus cuniculus) were identified as a major reservoir of C. burnetii and it is possible that they play a similar role in Australia. In absence of commercial kit available for rabbit, the Thermo Fisher - PrioCHECK™ Ruminant Q fever Ab Plate Kit was adapted to successfully screen rabbits population in Europe. However, this assay is not accessible in Australia and we assessed the equivalency of two commercially available kits in Australia – IDEXX - CHEKIT Q Fever Antibody ELISA kit and IDVet - ID Screen® Q Fever Indirect Multi-species with the Thermo Fisher kit (reference kit). RESULTS: A total of 94 rabbit sera were screened by all three ELISA kits using the same confirmed positive and negative controls. While the IDEXX kit failed to agree the other two assays (concordance correlation coefficient, r(b) < 0.77), IDVet kit showed satisfactory equivalency with Thermo Fisher (r(b) = 0.927). CONCLUSION: IDvet kit provides the best alternative for Thermo Fisher in the detection of C. burnetii specific antibodies in rabbits in Australia. Further trials are required to confirm these preliminary results due to the low seroprevalence of Coxiella burnetii observed in the study sera. BioMed Central 2020-08-28 /pmc/articles/PMC7456029/ /pubmed/32859195 http://dx.doi.org/10.1186/s12917-020-02526-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Caraguel, Charles
Bassett, Sarah
González-Barrio, David
Elsworth, Peter
Chaber, Anne-Lise
Comparison of three serological tests for the detection of Coxiella burnetii specific antibodies in European wild rabbits
title Comparison of three serological tests for the detection of Coxiella burnetii specific antibodies in European wild rabbits
title_full Comparison of three serological tests for the detection of Coxiella burnetii specific antibodies in European wild rabbits
title_fullStr Comparison of three serological tests for the detection of Coxiella burnetii specific antibodies in European wild rabbits
title_full_unstemmed Comparison of three serological tests for the detection of Coxiella burnetii specific antibodies in European wild rabbits
title_short Comparison of three serological tests for the detection of Coxiella burnetii specific antibodies in European wild rabbits
title_sort comparison of three serological tests for the detection of coxiella burnetii specific antibodies in european wild rabbits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456029/
https://www.ncbi.nlm.nih.gov/pubmed/32859195
http://dx.doi.org/10.1186/s12917-020-02526-w
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