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Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer

cir-ITCH, a well-known tumor-suppressive circular RNA, plays a critical role in different cancers. However, its expression and functional role in prostate cancer (PCa) are unclear. Herein, we explored the potential mechanism and tumor-inhibiting role of cir-ITCH in PCa. Using reverse transcriptase p...

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Autores principales: Li, Shoubin, Yu, Chunhong, Zhang, Yunxia, Liu, Junjiang, Jia, Yi, Sun, Fuzhen, Zhang, Panying, Li, Jingpo, Guo, Liuxiong, Xiao, Helong, Gao, Fei, Deng, Xinna, Cai, Ziqi, Cai, Jianhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456474/
https://www.ncbi.nlm.nih.gov/pubmed/32904490
http://dx.doi.org/10.1155/2020/7586521
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author Li, Shoubin
Yu, Chunhong
Zhang, Yunxia
Liu, Junjiang
Jia, Yi
Sun, Fuzhen
Zhang, Panying
Li, Jingpo
Guo, Liuxiong
Xiao, Helong
Gao, Fei
Deng, Xinna
Cai, Ziqi
Cai, Jianhui
author_facet Li, Shoubin
Yu, Chunhong
Zhang, Yunxia
Liu, Junjiang
Jia, Yi
Sun, Fuzhen
Zhang, Panying
Li, Jingpo
Guo, Liuxiong
Xiao, Helong
Gao, Fei
Deng, Xinna
Cai, Ziqi
Cai, Jianhui
author_sort Li, Shoubin
collection PubMed
description cir-ITCH, a well-known tumor-suppressive circular RNA, plays a critical role in different cancers. However, its expression and functional role in prostate cancer (PCa) are unclear. Herein, we explored the potential mechanism and tumor-inhibiting role of cir-ITCH in PCa. Using reverse transcriptase polymerase chain reaction assay, we analyzed the expression of cir-ITCH in PCa and paired adjacent nontumor tissue samples resected during surgical operation, as well as in two cell lines of human PCa (LNCaP and PC-3) and the immortalized normal prostate epithelial cell line (RWPE-1). Cell viability and migration of PCa cell lines were evaluated using CCK-8 and wound-healing assays. Expression of key proteins of the Wnt/β-catenin and PI3K/AKT/mTOR pathways was detected using western blotting. We found that cir-ITCH expression was typically downregulated in the tissues and cell lines of PCa compared to that in the peritumoral tissue and in RWPE-1 cells, respectively. The results showed that cir-ITCH overexpression significantly inhibits the proliferation, migration, and invasion of human PCa cells and that reciprocal inhibition of expression occurred between cir-ITCH and miR-17. Proteins in the Wnt/β-catenin and PI3K/AKT/mTOR pathways were downregulated by overexpression of cir-ITCH both in androgen receptor-positive LNCaP cells and androgen receptor-negative PC-3 cells. Taken together, these data demonstrated that cir-ITCH plays a tumor-suppressive role in human PCa cells, partly through the Wnt/β-catenin and PI3K/AKT/mTOR pathways. Thus, cir-ITCH may serve as a novel therapeutic target for the treatment of PCa, especially castration-resistant prostate cancer.
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spelling pubmed-74564742020-09-03 Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer Li, Shoubin Yu, Chunhong Zhang, Yunxia Liu, Junjiang Jia, Yi Sun, Fuzhen Zhang, Panying Li, Jingpo Guo, Liuxiong Xiao, Helong Gao, Fei Deng, Xinna Cai, Ziqi Cai, Jianhui Biomed Res Int Research Article cir-ITCH, a well-known tumor-suppressive circular RNA, plays a critical role in different cancers. However, its expression and functional role in prostate cancer (PCa) are unclear. Herein, we explored the potential mechanism and tumor-inhibiting role of cir-ITCH in PCa. Using reverse transcriptase polymerase chain reaction assay, we analyzed the expression of cir-ITCH in PCa and paired adjacent nontumor tissue samples resected during surgical operation, as well as in two cell lines of human PCa (LNCaP and PC-3) and the immortalized normal prostate epithelial cell line (RWPE-1). Cell viability and migration of PCa cell lines were evaluated using CCK-8 and wound-healing assays. Expression of key proteins of the Wnt/β-catenin and PI3K/AKT/mTOR pathways was detected using western blotting. We found that cir-ITCH expression was typically downregulated in the tissues and cell lines of PCa compared to that in the peritumoral tissue and in RWPE-1 cells, respectively. The results showed that cir-ITCH overexpression significantly inhibits the proliferation, migration, and invasion of human PCa cells and that reciprocal inhibition of expression occurred between cir-ITCH and miR-17. Proteins in the Wnt/β-catenin and PI3K/AKT/mTOR pathways were downregulated by overexpression of cir-ITCH both in androgen receptor-positive LNCaP cells and androgen receptor-negative PC-3 cells. Taken together, these data demonstrated that cir-ITCH plays a tumor-suppressive role in human PCa cells, partly through the Wnt/β-catenin and PI3K/AKT/mTOR pathways. Thus, cir-ITCH may serve as a novel therapeutic target for the treatment of PCa, especially castration-resistant prostate cancer. Hindawi 2020-08-20 /pmc/articles/PMC7456474/ /pubmed/32904490 http://dx.doi.org/10.1155/2020/7586521 Text en Copyright © 2020 Shoubin Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Shoubin
Yu, Chunhong
Zhang, Yunxia
Liu, Junjiang
Jia, Yi
Sun, Fuzhen
Zhang, Panying
Li, Jingpo
Guo, Liuxiong
Xiao, Helong
Gao, Fei
Deng, Xinna
Cai, Ziqi
Cai, Jianhui
Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer
title Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer
title_full Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer
title_fullStr Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer
title_full_unstemmed Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer
title_short Circular RNA cir-ITCH Is a Potential Therapeutic Target for the Treatment of Castration-Resistant Prostate Cancer
title_sort circular rna cir-itch is a potential therapeutic target for the treatment of castration-resistant prostate cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456474/
https://www.ncbi.nlm.nih.gov/pubmed/32904490
http://dx.doi.org/10.1155/2020/7586521
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