A Cell-Based Method to Detect Agonist and Antagonist Activities of Endocrine-Disrupting Chemicals on GPER

Endocrine-disrupting chemicals (EDCs) are exogenous compounds that impact endogenous hormonal systems, resulting in adverse health effects. These chemicals can exert their actions by interfering with several pathways. Simple biological systems to determine whether EDCs act positively or negatively o...

Descripción completa

Detalles Bibliográficos
Autores principales: Périan, Séverine, Cerutti, Catherine, Forcet, Christelle, Tribollet, Violaine, Vanacker, Jean-Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Endocrinology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456940/
https://www.ncbi.nlm.nih.gov/pubmed/32922363
http://dx.doi.org/10.3389/fendo.2020.00547
_version_ 1783575899816329216
author Périan, Séverine
Cerutti, Catherine
Forcet, Christelle
Tribollet, Violaine
Vanacker, Jean-Marc
author_facet Périan, Séverine
Cerutti, Catherine
Forcet, Christelle
Tribollet, Violaine
Vanacker, Jean-Marc
author_sort Périan, Séverine
collection PubMed
description Endocrine-disrupting chemicals (EDCs) are exogenous compounds that impact endogenous hormonal systems, resulting in adverse health effects. These chemicals can exert their actions by interfering with several pathways. Simple biological systems to determine whether EDCs act positively or negatively on a given receptor are often lacking. Here we describe a low-to-middle throughput method to screen the agonist/antagonist potential of EDCs specifically on the GPER membrane estrogen receptor. Application of this assay to 23 candidate EDCs from different chemical families reveals the existence of six agonists and six antagonists.
format Online
Article
Text
id pubmed-7456940
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-74569402020-09-11 A Cell-Based Method to Detect Agonist and Antagonist Activities of Endocrine-Disrupting Chemicals on GPER Périan, Séverine Cerutti, Catherine Forcet, Christelle Tribollet, Violaine Vanacker, Jean-Marc Front Endocrinol (Lausanne) Endocrinology Endocrine-disrupting chemicals (EDCs) are exogenous compounds that impact endogenous hormonal systems, resulting in adverse health effects. These chemicals can exert their actions by interfering with several pathways. Simple biological systems to determine whether EDCs act positively or negatively on a given receptor are often lacking. Here we describe a low-to-middle throughput method to screen the agonist/antagonist potential of EDCs specifically on the GPER membrane estrogen receptor. Application of this assay to 23 candidate EDCs from different chemical families reveals the existence of six agonists and six antagonists. Frontiers Media S.A. 2020-08-14 /pmc/articles/PMC7456940/ /pubmed/32922363 http://dx.doi.org/10.3389/fendo.2020.00547 Text en Copyright © 2020 Périan, Cerutti, Forcet, Tribollet and Vanacker. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Périan, Séverine
Cerutti, Catherine
Forcet, Christelle
Tribollet, Violaine
Vanacker, Jean-Marc
A Cell-Based Method to Detect Agonist and Antagonist Activities of Endocrine-Disrupting Chemicals on GPER
title A Cell-Based Method to Detect Agonist and Antagonist Activities of Endocrine-Disrupting Chemicals on GPER
title_full A Cell-Based Method to Detect Agonist and Antagonist Activities of Endocrine-Disrupting Chemicals on GPER
title_fullStr A Cell-Based Method to Detect Agonist and Antagonist Activities of Endocrine-Disrupting Chemicals on GPER
title_full_unstemmed A Cell-Based Method to Detect Agonist and Antagonist Activities of Endocrine-Disrupting Chemicals on GPER
title_short A Cell-Based Method to Detect Agonist and Antagonist Activities of Endocrine-Disrupting Chemicals on GPER
title_sort cell-based method to detect agonist and antagonist activities of endocrine-disrupting chemicals on gper
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456940/
https://www.ncbi.nlm.nih.gov/pubmed/32922363
http://dx.doi.org/10.3389/fendo.2020.00547
work_keys_str_mv AT perianseverine acellbasedmethodtodetectagonistandantagonistactivitiesofendocrinedisruptingchemicalsongper
AT cerutticatherine acellbasedmethodtodetectagonistandantagonistactivitiesofendocrinedisruptingchemicalsongper
AT forcetchristelle acellbasedmethodtodetectagonistandantagonistactivitiesofendocrinedisruptingchemicalsongper
AT tribolletviolaine acellbasedmethodtodetectagonistandantagonistactivitiesofendocrinedisruptingchemicalsongper
AT vanackerjeanmarc acellbasedmethodtodetectagonistandantagonistactivitiesofendocrinedisruptingchemicalsongper
AT perianseverine cellbasedmethodtodetectagonistandantagonistactivitiesofendocrinedisruptingchemicalsongper
AT cerutticatherine cellbasedmethodtodetectagonistandantagonistactivitiesofendocrinedisruptingchemicalsongper
AT forcetchristelle cellbasedmethodtodetectagonistandantagonistactivitiesofendocrinedisruptingchemicalsongper
AT tribolletviolaine cellbasedmethodtodetectagonistandantagonistactivitiesofendocrinedisruptingchemicalsongper
AT vanackerjeanmarc cellbasedmethodtodetectagonistandantagonistactivitiesofendocrinedisruptingchemicalsongper

Ejemplares similares