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Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression
Molecular biotechnology has made it possible to explore the potential of plants for different purposes. The 3’ regulatory regions have a great diversity of cis-regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457121/ https://www.ncbi.nlm.nih.gov/pubmed/32922424 http://dx.doi.org/10.3389/fpls.2020.01252 |
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author | Bernardes, Willian Souza Menossi, Marcelo |
author_facet | Bernardes, Willian Souza Menossi, Marcelo |
author_sort | Bernardes, Willian Souza |
collection | PubMed |
description | Molecular biotechnology has made it possible to explore the potential of plants for different purposes. The 3’ regulatory regions have a great diversity of cis-regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. A complex interaction between the cleavage and polyadenylation molecular complex and cis-elements determine the polyadenylation site, which may result in the choice of non-canonical sites, resulting in alternative polyadenylation events, involved in the regulation of more than 80% of the genes expressed in plants. In addition, after transcription, a wide array of RNA-binding proteins interacts with cis-acting elements located mainly in the 3’ untranslated region, determining the fate of mRNAs in eukaryotic cells. Although a small number of 3’ regulatory regions have been identified and validated so far, many studies have shown that plant 3’ regulatory regions have a higher potential to regulate gene expression in plants compared to widely used 3’ regulatory regions, such as NOS and OCS from Agrobacterium tumefaciens and 35S from cauliflower mosaic virus. In this review, we discuss the role of 3’ regulatory regions in gene expression, and the superior potential that plant 3’ regulatory regions have compared to NOS, OCS and 35S 3’ regulatory regions. |
format | Online Article Text |
id | pubmed-7457121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74571212020-09-11 Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression Bernardes, Willian Souza Menossi, Marcelo Front Plant Sci Plant Science Molecular biotechnology has made it possible to explore the potential of plants for different purposes. The 3’ regulatory regions have a great diversity of cis-regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. A complex interaction between the cleavage and polyadenylation molecular complex and cis-elements determine the polyadenylation site, which may result in the choice of non-canonical sites, resulting in alternative polyadenylation events, involved in the regulation of more than 80% of the genes expressed in plants. In addition, after transcription, a wide array of RNA-binding proteins interacts with cis-acting elements located mainly in the 3’ untranslated region, determining the fate of mRNAs in eukaryotic cells. Although a small number of 3’ regulatory regions have been identified and validated so far, many studies have shown that plant 3’ regulatory regions have a higher potential to regulate gene expression in plants compared to widely used 3’ regulatory regions, such as NOS and OCS from Agrobacterium tumefaciens and 35S from cauliflower mosaic virus. In this review, we discuss the role of 3’ regulatory regions in gene expression, and the superior potential that plant 3’ regulatory regions have compared to NOS, OCS and 35S 3’ regulatory regions. Frontiers Media S.A. 2020-08-14 /pmc/articles/PMC7457121/ /pubmed/32922424 http://dx.doi.org/10.3389/fpls.2020.01252 Text en Copyright © 2020 Bernardes and Menossi http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Bernardes, Willian Souza Menossi, Marcelo Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression |
title | Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression |
title_full | Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression |
title_fullStr | Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression |
title_full_unstemmed | Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression |
title_short | Plant 3’ Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression |
title_sort | plant 3’ regulatory regions from mrna-encoding genes and their uses to modulate expression |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457121/ https://www.ncbi.nlm.nih.gov/pubmed/32922424 http://dx.doi.org/10.3389/fpls.2020.01252 |
work_keys_str_mv | AT bernardeswilliansouza plant3regulatoryregionsfrommrnaencodinggenesandtheirusestomodulateexpression AT menossimarcelo plant3regulatoryregionsfrommrnaencodinggenesandtheirusestomodulateexpression |