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HLA class II donor specific antibodies are associated with graft cirrhosis after liver transplant independent of the mean fluorescence intensity level

BACKGROUND: The importance of donor-specific antibodies (DSA) after liver transplantation (LT) for graft and patient survival is an ongoing controversy. So far it has not been elucidated when and in how far DSA are harmful for graft and patient survival. Therefore, we had the aim to investigate the...

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Autores principales: Willuweit, Katharina, Frey, Alexandra, Bieniek, Lisa, Heinold, Andreas, Büchter, Matthias, Horn, Peter A., Wedemeyer, Heiner, Herzer, Kerstin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457295/
https://www.ncbi.nlm.nih.gov/pubmed/32854625
http://dx.doi.org/10.1186/s12876-020-01427-4
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author Willuweit, Katharina
Frey, Alexandra
Bieniek, Lisa
Heinold, Andreas
Büchter, Matthias
Horn, Peter A.
Wedemeyer, Heiner
Herzer, Kerstin
author_facet Willuweit, Katharina
Frey, Alexandra
Bieniek, Lisa
Heinold, Andreas
Büchter, Matthias
Horn, Peter A.
Wedemeyer, Heiner
Herzer, Kerstin
author_sort Willuweit, Katharina
collection PubMed
description BACKGROUND: The importance of donor-specific antibodies (DSA) after liver transplantation (LT) for graft and patient survival is an ongoing controversy. So far it has not been elucidated when and in how far DSA are harmful for graft and patient survival. Therefore, we had the aim to investigate the association of DSA with complications after LT. METHODS: Data of 430 LT recipients were collected and statistically analyzed. Detection of HLA antibodies (Ab) was performed by Luminex assay. RESULTS: DSA were detected in 81 patients (18.8%). These were mainly HLA class II Ab (81.5%). HLA class II Ab show a higher MFI (median: 5.300) compared to HLA class I Ab (median: 2.300). There is no association between MFI levels and development of complications after LT. However, cirrhosis occurred significantly more often in DSA positive patients (18%) than in patients without detectable DSA (9%, P = 0.027). All DSA positive patients with cirrhosis of the graft showed HLA class II antibodies (OR: 3.028; 95% CI: 1.51–6.075; P = 0.002). CONCLUSION: Occurrence of HLA class II DSA after LT is associated with graft cirrhosis and may indicate a higher risk to develop graft damage independent on MFI and requires an individualized risk management.
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spelling pubmed-74572952020-08-31 HLA class II donor specific antibodies are associated with graft cirrhosis after liver transplant independent of the mean fluorescence intensity level Willuweit, Katharina Frey, Alexandra Bieniek, Lisa Heinold, Andreas Büchter, Matthias Horn, Peter A. Wedemeyer, Heiner Herzer, Kerstin BMC Gastroenterol Research Article BACKGROUND: The importance of donor-specific antibodies (DSA) after liver transplantation (LT) for graft and patient survival is an ongoing controversy. So far it has not been elucidated when and in how far DSA are harmful for graft and patient survival. Therefore, we had the aim to investigate the association of DSA with complications after LT. METHODS: Data of 430 LT recipients were collected and statistically analyzed. Detection of HLA antibodies (Ab) was performed by Luminex assay. RESULTS: DSA were detected in 81 patients (18.8%). These were mainly HLA class II Ab (81.5%). HLA class II Ab show a higher MFI (median: 5.300) compared to HLA class I Ab (median: 2.300). There is no association between MFI levels and development of complications after LT. However, cirrhosis occurred significantly more often in DSA positive patients (18%) than in patients without detectable DSA (9%, P = 0.027). All DSA positive patients with cirrhosis of the graft showed HLA class II antibodies (OR: 3.028; 95% CI: 1.51–6.075; P = 0.002). CONCLUSION: Occurrence of HLA class II DSA after LT is associated with graft cirrhosis and may indicate a higher risk to develop graft damage independent on MFI and requires an individualized risk management. BioMed Central 2020-08-27 /pmc/articles/PMC7457295/ /pubmed/32854625 http://dx.doi.org/10.1186/s12876-020-01427-4 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Willuweit, Katharina
Frey, Alexandra
Bieniek, Lisa
Heinold, Andreas
Büchter, Matthias
Horn, Peter A.
Wedemeyer, Heiner
Herzer, Kerstin
HLA class II donor specific antibodies are associated with graft cirrhosis after liver transplant independent of the mean fluorescence intensity level
title HLA class II donor specific antibodies are associated with graft cirrhosis after liver transplant independent of the mean fluorescence intensity level
title_full HLA class II donor specific antibodies are associated with graft cirrhosis after liver transplant independent of the mean fluorescence intensity level
title_fullStr HLA class II donor specific antibodies are associated with graft cirrhosis after liver transplant independent of the mean fluorescence intensity level
title_full_unstemmed HLA class II donor specific antibodies are associated with graft cirrhosis after liver transplant independent of the mean fluorescence intensity level
title_short HLA class II donor specific antibodies are associated with graft cirrhosis after liver transplant independent of the mean fluorescence intensity level
title_sort hla class ii donor specific antibodies are associated with graft cirrhosis after liver transplant independent of the mean fluorescence intensity level
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457295/
https://www.ncbi.nlm.nih.gov/pubmed/32854625
http://dx.doi.org/10.1186/s12876-020-01427-4
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