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Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion
The molecular basis for the severity and rapid spread of the COVID-19 disease caused by SARS-CoV-2 is largely unknown. ORF8 is a rapidly evolving accessory protein that has been proposed to interfere with immune responses. The crystal structure of SARS-CoV-2 ORF8 was determined at 2.04 Å resolution...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457612/ https://www.ncbi.nlm.nih.gov/pubmed/32869027 http://dx.doi.org/10.1101/2020.08.27.270637 |
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author | Flower, Thomas G. Buffalo, Cosmo Z. Hooy, Richard M. Allaire, Marc Ren, Xuefeng Hurley, James H. |
author_facet | Flower, Thomas G. Buffalo, Cosmo Z. Hooy, Richard M. Allaire, Marc Ren, Xuefeng Hurley, James H. |
author_sort | Flower, Thomas G. |
collection | PubMed |
description | The molecular basis for the severity and rapid spread of the COVID-19 disease caused by SARS-CoV-2 is largely unknown. ORF8 is a rapidly evolving accessory protein that has been proposed to interfere with immune responses. The crystal structure of SARS-CoV-2 ORF8 was determined at 2.04 Å resolution by x-ray crystallography. The structure reveals a ~60 residue core similar to SARS-CoV ORF7a with the addition of two dimerization interfaces unique to SARS-CoV-2 ORF8. A covalent disulfide-linked dimer is formed through an N-terminal sequence specific to SARS-CoV-2, while a separate non-covalent interface is formed by another SARS-CoV-2-specific sequence, (73)YIDI(76). Together the presence of these interfaces shows how SARS-CoV-2 ORF8 can form unique large-scale assemblies not possible for SARS-CoV, potentially mediating unique immune suppression and evasion activities. |
format | Online Article Text |
id | pubmed-7457612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-74576122020-09-01 Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion Flower, Thomas G. Buffalo, Cosmo Z. Hooy, Richard M. Allaire, Marc Ren, Xuefeng Hurley, James H. bioRxiv Article The molecular basis for the severity and rapid spread of the COVID-19 disease caused by SARS-CoV-2 is largely unknown. ORF8 is a rapidly evolving accessory protein that has been proposed to interfere with immune responses. The crystal structure of SARS-CoV-2 ORF8 was determined at 2.04 Å resolution by x-ray crystallography. The structure reveals a ~60 residue core similar to SARS-CoV ORF7a with the addition of two dimerization interfaces unique to SARS-CoV-2 ORF8. A covalent disulfide-linked dimer is formed through an N-terminal sequence specific to SARS-CoV-2, while a separate non-covalent interface is formed by another SARS-CoV-2-specific sequence, (73)YIDI(76). Together the presence of these interfaces shows how SARS-CoV-2 ORF8 can form unique large-scale assemblies not possible for SARS-CoV, potentially mediating unique immune suppression and evasion activities. Cold Spring Harbor Laboratory 2020-08-27 /pmc/articles/PMC7457612/ /pubmed/32869027 http://dx.doi.org/10.1101/2020.08.27.270637 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/It is made available under a CC-BY-NC-ND 4.0 International license (http://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Article Flower, Thomas G. Buffalo, Cosmo Z. Hooy, Richard M. Allaire, Marc Ren, Xuefeng Hurley, James H. Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion |
title | Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion |
title_full | Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion |
title_fullStr | Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion |
title_full_unstemmed | Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion |
title_short | Structure of SARS-CoV-2 ORF8, a rapidly evolving coronavirus protein implicated in immune evasion |
title_sort | structure of sars-cov-2 orf8, a rapidly evolving coronavirus protein implicated in immune evasion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457612/ https://www.ncbi.nlm.nih.gov/pubmed/32869027 http://dx.doi.org/10.1101/2020.08.27.270637 |
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