Temporal Detection and Phylogenetic Assessment of SARS-CoV-2 in Municipal Wastewater

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SARS-CoV-2 has recently been detected in feces, which indicates that wastewater may be used to monitor viral prevalence in the community. Here, we use RT-qPCR to monitor wastewater for SARS-CoV-2 RNA over a 74-day time course. We show that changes in SARS-CoV-2 RNA concentrations follow symptom onse...

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Detalles Bibliográficos
Autores principales: Nemudryi, Artem, Nemudraia, Anna, Wiegand, Tanner, Surya, Kevin, Buyukyoruk, Murat, Cicha, Calvin, Vanderwood, Karl K., Wilkinson, Royce, Wiedenheft, Blake
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457911/
https://www.ncbi.nlm.nih.gov/pubmed/32904687
http://dx.doi.org/10.1016/j.xcrm.2020.100098
Descripción
Sumario:SARS-CoV-2 has recently been detected in feces, which indicates that wastewater may be used to monitor viral prevalence in the community. Here, we use RT-qPCR to monitor wastewater for SARS-CoV-2 RNA over a 74-day time course. We show that changes in SARS-CoV-2 RNA concentrations follow symptom onset gathered by retrospective interview of patients but precedes clinical test results. In addition, we determine a nearly complete (98.5%) SARS-CoV-2 genome sequence from wastewater and use phylogenetic analysis to infer viral ancestry. Collectively, this work demonstrates how wastewater can be used as a proxy to monitor viral prevalence in the community and how genome sequencing can be used for genotyping viral strains circulating in a community.

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