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A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers
Liver cancer stem cells (L‐CSCs) are considered to be an important therapeutic target for hepatocellular carcinoma (HCC). This study provides a new in vitro long‐term culture model for a specific subpopulation of L‐CSCs enriched by cell surface markers. We combined CD13, CD133 and EpCAM to selective...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459405/ https://www.ncbi.nlm.nih.gov/pubmed/32662250 http://dx.doi.org/10.1002/2211-5463.12932 |
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author | Zhang, Biao Wang, Hai‐Yang Wang, Dong‐Xing Zeng, Quan Fan, Zeng Xi, Jia‐Fei Nan, Xue He, Li‐Juan Zhou, Jun‐Nian Pei, Xue‐Tao Yue, Wen |
author_facet | Zhang, Biao Wang, Hai‐Yang Wang, Dong‐Xing Zeng, Quan Fan, Zeng Xi, Jia‐Fei Nan, Xue He, Li‐Juan Zhou, Jun‐Nian Pei, Xue‐Tao Yue, Wen |
author_sort | Zhang, Biao |
collection | PubMed |
description | Liver cancer stem cells (L‐CSCs) are considered to be an important therapeutic target for hepatocellular carcinoma (HCC). This study provides a new in vitro long‐term culture model for a specific subpopulation of L‐CSCs enriched by cell surface markers. We combined CD13, CD133 and EpCAM to selectively enrich L‐CSCs, which we then cultured in modified chemically defined medium. The enriched L‐CSCs exhibited enhanced proliferation, self‐renewal and long‐term clonal maintenance ability as compared with non‐CSCs. Compared with wild‐type hepatocellular carcinoma, the expression of stemness surface markers, oncogenes, drug resistance and tumorigenicity in enriched L‐CSCs was significantly increased. In summary, the subpopulation of L‐CSCs still maintains cancer stem cell‐related phenotypes after 14 days of culture. |
format | Online Article Text |
id | pubmed-7459405 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74594052020-09-03 A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers Zhang, Biao Wang, Hai‐Yang Wang, Dong‐Xing Zeng, Quan Fan, Zeng Xi, Jia‐Fei Nan, Xue He, Li‐Juan Zhou, Jun‐Nian Pei, Xue‐Tao Yue, Wen FEBS Open Bio Research Articles Liver cancer stem cells (L‐CSCs) are considered to be an important therapeutic target for hepatocellular carcinoma (HCC). This study provides a new in vitro long‐term culture model for a specific subpopulation of L‐CSCs enriched by cell surface markers. We combined CD13, CD133 and EpCAM to selectively enrich L‐CSCs, which we then cultured in modified chemically defined medium. The enriched L‐CSCs exhibited enhanced proliferation, self‐renewal and long‐term clonal maintenance ability as compared with non‐CSCs. Compared with wild‐type hepatocellular carcinoma, the expression of stemness surface markers, oncogenes, drug resistance and tumorigenicity in enriched L‐CSCs was significantly increased. In summary, the subpopulation of L‐CSCs still maintains cancer stem cell‐related phenotypes after 14 days of culture. John Wiley and Sons Inc. 2020-07-31 /pmc/articles/PMC7459405/ /pubmed/32662250 http://dx.doi.org/10.1002/2211-5463.12932 Text en © 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Zhang, Biao Wang, Hai‐Yang Wang, Dong‐Xing Zeng, Quan Fan, Zeng Xi, Jia‐Fei Nan, Xue He, Li‐Juan Zhou, Jun‐Nian Pei, Xue‐Tao Yue, Wen A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers |
title | A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers |
title_full | A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers |
title_fullStr | A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers |
title_full_unstemmed | A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers |
title_short | A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers |
title_sort | new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459405/ https://www.ncbi.nlm.nih.gov/pubmed/32662250 http://dx.doi.org/10.1002/2211-5463.12932 |
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