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A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers

Liver cancer stem cells (L‐CSCs) are considered to be an important therapeutic target for hepatocellular carcinoma (HCC). This study provides a new in vitro long‐term culture model for a specific subpopulation of L‐CSCs enriched by cell surface markers. We combined CD13, CD133 and EpCAM to selective...

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Autores principales: Zhang, Biao, Wang, Hai‐Yang, Wang, Dong‐Xing, Zeng, Quan, Fan, Zeng, Xi, Jia‐Fei, Nan, Xue, He, Li‐Juan, Zhou, Jun‐Nian, Pei, Xue‐Tao, Yue, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459405/
https://www.ncbi.nlm.nih.gov/pubmed/32662250
http://dx.doi.org/10.1002/2211-5463.12932
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author Zhang, Biao
Wang, Hai‐Yang
Wang, Dong‐Xing
Zeng, Quan
Fan, Zeng
Xi, Jia‐Fei
Nan, Xue
He, Li‐Juan
Zhou, Jun‐Nian
Pei, Xue‐Tao
Yue, Wen
author_facet Zhang, Biao
Wang, Hai‐Yang
Wang, Dong‐Xing
Zeng, Quan
Fan, Zeng
Xi, Jia‐Fei
Nan, Xue
He, Li‐Juan
Zhou, Jun‐Nian
Pei, Xue‐Tao
Yue, Wen
author_sort Zhang, Biao
collection PubMed
description Liver cancer stem cells (L‐CSCs) are considered to be an important therapeutic target for hepatocellular carcinoma (HCC). This study provides a new in vitro long‐term culture model for a specific subpopulation of L‐CSCs enriched by cell surface markers. We combined CD13, CD133 and EpCAM to selectively enrich L‐CSCs, which we then cultured in modified chemically defined medium. The enriched L‐CSCs exhibited enhanced proliferation, self‐renewal and long‐term clonal maintenance ability as compared with non‐CSCs. Compared with wild‐type hepatocellular carcinoma, the expression of stemness surface markers, oncogenes, drug resistance and tumorigenicity in enriched L‐CSCs was significantly increased. In summary, the subpopulation of L‐CSCs still maintains cancer stem cell‐related phenotypes after 14 days of culture.
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spelling pubmed-74594052020-09-03 A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers Zhang, Biao Wang, Hai‐Yang Wang, Dong‐Xing Zeng, Quan Fan, Zeng Xi, Jia‐Fei Nan, Xue He, Li‐Juan Zhou, Jun‐Nian Pei, Xue‐Tao Yue, Wen FEBS Open Bio Research Articles Liver cancer stem cells (L‐CSCs) are considered to be an important therapeutic target for hepatocellular carcinoma (HCC). This study provides a new in vitro long‐term culture model for a specific subpopulation of L‐CSCs enriched by cell surface markers. We combined CD13, CD133 and EpCAM to selectively enrich L‐CSCs, which we then cultured in modified chemically defined medium. The enriched L‐CSCs exhibited enhanced proliferation, self‐renewal and long‐term clonal maintenance ability as compared with non‐CSCs. Compared with wild‐type hepatocellular carcinoma, the expression of stemness surface markers, oncogenes, drug resistance and tumorigenicity in enriched L‐CSCs was significantly increased. In summary, the subpopulation of L‐CSCs still maintains cancer stem cell‐related phenotypes after 14 days of culture. John Wiley and Sons Inc. 2020-07-31 /pmc/articles/PMC7459405/ /pubmed/32662250 http://dx.doi.org/10.1002/2211-5463.12932 Text en © 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Zhang, Biao
Wang, Hai‐Yang
Wang, Dong‐Xing
Zeng, Quan
Fan, Zeng
Xi, Jia‐Fei
Nan, Xue
He, Li‐Juan
Zhou, Jun‐Nian
Pei, Xue‐Tao
Yue, Wen
A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers
title A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers
title_full A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers
title_fullStr A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers
title_full_unstemmed A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers
title_short A new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers
title_sort new protocol for long‐term culture of a specific subpopulation of liver cancer stem cells enriched by cell surface markers
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459405/
https://www.ncbi.nlm.nih.gov/pubmed/32662250
http://dx.doi.org/10.1002/2211-5463.12932
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