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Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage

Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis...

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Autores principales: Montesinos, Juan Carlos, Abuzeineh, Anas, Kopf, Aglaja, Juanes‐Garcia, Alba, Ötvös, Krisztina, Petrášek, Jan, Sixt, Michael, Benková, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459425/
https://www.ncbi.nlm.nih.gov/pubmed/32667089
http://dx.doi.org/10.15252/embj.2019104238
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author Montesinos, Juan Carlos
Abuzeineh, Anas
Kopf, Aglaja
Juanes‐Garcia, Alba
Ötvös, Krisztina
Petrášek, Jan
Sixt, Michael
Benková, Eva
author_facet Montesinos, Juan Carlos
Abuzeineh, Anas
Kopf, Aglaja
Juanes‐Garcia, Alba
Ötvös, Krisztina
Petrášek, Jan
Sixt, Michael
Benková, Eva
author_sort Montesinos, Juan Carlos
collection PubMed
description Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis. Here, we show that the plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton during cell differentiation and counteracts microtubular rearrangements driven by the hormone auxin. The endogenous upward gradient of cytokinin activity along the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust rearrangements of the microtubule cytoskeleton in epidermal cells progressing from the proliferative to the differentiation stage. Controlled increases in cytokinin activity result in premature re‐organization of the microtubule network from transversal to an oblique disposition in cells prior to their differentiation, whereas attenuated hormone perception delays cytoskeleton conversion into a configuration typical for differentiated cells. Intriguingly, cytokinin can interfere with microtubules also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive control pathway for the microtubular cytoskeleton may be at least partially conserved between plant and animal cells.
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spelling pubmed-74594252020-09-03 Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage Montesinos, Juan Carlos Abuzeineh, Anas Kopf, Aglaja Juanes‐Garcia, Alba Ötvös, Krisztina Petrášek, Jan Sixt, Michael Benková, Eva EMBO J Articles Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis. Here, we show that the plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton during cell differentiation and counteracts microtubular rearrangements driven by the hormone auxin. The endogenous upward gradient of cytokinin activity along the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust rearrangements of the microtubule cytoskeleton in epidermal cells progressing from the proliferative to the differentiation stage. Controlled increases in cytokinin activity result in premature re‐organization of the microtubule network from transversal to an oblique disposition in cells prior to their differentiation, whereas attenuated hormone perception delays cytoskeleton conversion into a configuration typical for differentiated cells. Intriguingly, cytokinin can interfere with microtubules also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive control pathway for the microtubular cytoskeleton may be at least partially conserved between plant and animal cells. John Wiley and Sons Inc. 2020-07-15 2020-09-01 /pmc/articles/PMC7459425/ /pubmed/32667089 http://dx.doi.org/10.15252/embj.2019104238 Text en © 2020 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Montesinos, Juan Carlos
Abuzeineh, Anas
Kopf, Aglaja
Juanes‐Garcia, Alba
Ötvös, Krisztina
Petrášek, Jan
Sixt, Michael
Benková, Eva
Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage
title Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage
title_full Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage
title_fullStr Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage
title_full_unstemmed Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage
title_short Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage
title_sort phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459425/
https://www.ncbi.nlm.nih.gov/pubmed/32667089
http://dx.doi.org/10.15252/embj.2019104238
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