Changing Human Behavior to Improve Animal Welfare: A Longitudinal Investigation of Training Laboratory Animal Personnel about Heterospecific Play or “Rat Tickling”

SIMPLE SUMMARY: When laboratory rats are first handled, they can experience fear and stress, which negatively influences animal welfare. Rat tickling, a positive handling technique, can improve these outcomes. However, despite evidence for rat tickling’s animal welfare benefits, the technique is rarely implemented, in part because of a lack of training. Our purpose was to determine the effectiveness of two rat tickling training programs (as compared to a control treatment) on reported implementation, self-efficacy, knowledge, familiarity, and beliefs about rat tickling. After completing an initial survey, 96 laboratory animal personnel currently working with rats were assigned to receive online-only training, online + hands-on training, or no training (control condition). Participants received further surveys directly after training and 2 months later. Results showed that both online-only and online + hands-on training improved key outcomes for rat tickling (i.e., increased implementation, self-efficacy, knowledge, and familiarity rat tickling). Online + hands-on training had a few additional benefits (i.e., increased control beliefs and greater increases for self-efficacy and familiarity with rat tickling). Overall, these findings support the development of targeted interactive training programs to improve the implementation of potential welfare-enhancing techniques. ABSTRACT: Despite evidence for rat tickling’s animal welfare benefits, the technique is rarely implemented in part because of a lack of training. This study’s purpose was to determine the efficacy of online-only or online + hands-on training programs on key outcomes for rat tickling in comparison to a waitlist control condition. After completing a baseline survey, laboratory animal personnel currently working with rats in the United States were semi-randomized to receive online-only training (n = 30), online + hands-on training (n = 34), or waitlist control (n = 32). Participants received further surveys directly after training and 2 months later. Data were analyzed using general linear mixed models. At the 2-month follow-up compared to baseline, both training groups reported increased implementation, self-efficacy, knowledge, and familiarity of rat tickling while only the online + hands-on training participants reported increased control beliefs (while the waitlist group stayed the same). At the 2-month follow-up compared to the waitlist, hands-on training participants reported increased self-efficacy and familiarity with rat tickling. Overall, findings show that both online-only and online + hands-on training can improve key outcomes for rat tickling. Although online + hands-on training is slightly more effective, the interactive online-only training has the potential to improve widescale implementation of a welfare-enhancing technique.