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Molecular Characterization and Pathogenicity of Chicken Parvovirus (ChPV) in Specific Pathogen-Free Chicks Infected Experimentally

Chicken parvovirus (ChPV) is an agent frequently associated with runting stunting syndrome (RSS). This syndrome has been reported in association with ChPV in many countries, including Brazil; however, studies characterizing the virus on a molecular level are scarce, and ChPV pathogenicity in day-old...

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Autores principales: N. Nuñez, Luis Fabian, Santander-Parra, Silvana H., De la Torre, David I., de Sá, Lilian R. M., Buim, Marcos R., Astolfi-Ferreira, Claudete S., Piantino Ferreira, Antonio J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459526/
https://www.ncbi.nlm.nih.gov/pubmed/32722416
http://dx.doi.org/10.3390/pathogens9080606
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author N. Nuñez, Luis Fabian
Santander-Parra, Silvana H.
De la Torre, David I.
de Sá, Lilian R. M.
Buim, Marcos R.
Astolfi-Ferreira, Claudete S.
Piantino Ferreira, Antonio J.
author_facet N. Nuñez, Luis Fabian
Santander-Parra, Silvana H.
De la Torre, David I.
de Sá, Lilian R. M.
Buim, Marcos R.
Astolfi-Ferreira, Claudete S.
Piantino Ferreira, Antonio J.
author_sort N. Nuñez, Luis Fabian
collection PubMed
description Chicken parvovirus (ChPV) is an agent frequently associated with runting stunting syndrome (RSS). This syndrome has been reported in association with ChPV in many countries, including Brazil; however, studies characterizing the virus on a molecular level are scarce, and ChPV pathogenicity in day-old chicks remains unclear. The aim of the present work was to establish the molecular characteristics of ChPV, determine the pathogenicity of ChPV in SPF chicks and detect and quantify ChPV by qPCR in several tissues and chicks of different ages. The experimental challenge was performed at one day of age, and daily and weekly observations were performed and five birds from each experimental group (mock and infected birds) were euthanized to perform the different analysis. ChPV genome copies were detected and quantified by qPCR in gut, spleen, thymus, kidney, pancreas, proventriculus and bursa. Clinically, the infected group presented with diarrhea 24 h post-infection, which persisted until 42 days of age. The small intestine was distended, and its contents were aqueous and foamy. Enteritis and dilated crypts with cyst shapes were observed in intestinal segments. Acute pancreatitis associated with lymphocytic nodules, infiltrating lymphocytes and plasma cells between the pancreatic acinus was observed. Koch’s postulate was demonstrated and the genetic characterization of the VP1 gene showed that the Brazilian ChPV isolate belongs to the ChPV II group.
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spelling pubmed-74595262020-09-02 Molecular Characterization and Pathogenicity of Chicken Parvovirus (ChPV) in Specific Pathogen-Free Chicks Infected Experimentally N. Nuñez, Luis Fabian Santander-Parra, Silvana H. De la Torre, David I. de Sá, Lilian R. M. Buim, Marcos R. Astolfi-Ferreira, Claudete S. Piantino Ferreira, Antonio J. Pathogens Article Chicken parvovirus (ChPV) is an agent frequently associated with runting stunting syndrome (RSS). This syndrome has been reported in association with ChPV in many countries, including Brazil; however, studies characterizing the virus on a molecular level are scarce, and ChPV pathogenicity in day-old chicks remains unclear. The aim of the present work was to establish the molecular characteristics of ChPV, determine the pathogenicity of ChPV in SPF chicks and detect and quantify ChPV by qPCR in several tissues and chicks of different ages. The experimental challenge was performed at one day of age, and daily and weekly observations were performed and five birds from each experimental group (mock and infected birds) were euthanized to perform the different analysis. ChPV genome copies were detected and quantified by qPCR in gut, spleen, thymus, kidney, pancreas, proventriculus and bursa. Clinically, the infected group presented with diarrhea 24 h post-infection, which persisted until 42 days of age. The small intestine was distended, and its contents were aqueous and foamy. Enteritis and dilated crypts with cyst shapes were observed in intestinal segments. Acute pancreatitis associated with lymphocytic nodules, infiltrating lymphocytes and plasma cells between the pancreatic acinus was observed. Koch’s postulate was demonstrated and the genetic characterization of the VP1 gene showed that the Brazilian ChPV isolate belongs to the ChPV II group. MDPI 2020-07-25 /pmc/articles/PMC7459526/ /pubmed/32722416 http://dx.doi.org/10.3390/pathogens9080606 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
N. Nuñez, Luis Fabian
Santander-Parra, Silvana H.
De la Torre, David I.
de Sá, Lilian R. M.
Buim, Marcos R.
Astolfi-Ferreira, Claudete S.
Piantino Ferreira, Antonio J.
Molecular Characterization and Pathogenicity of Chicken Parvovirus (ChPV) in Specific Pathogen-Free Chicks Infected Experimentally
title Molecular Characterization and Pathogenicity of Chicken Parvovirus (ChPV) in Specific Pathogen-Free Chicks Infected Experimentally
title_full Molecular Characterization and Pathogenicity of Chicken Parvovirus (ChPV) in Specific Pathogen-Free Chicks Infected Experimentally
title_fullStr Molecular Characterization and Pathogenicity of Chicken Parvovirus (ChPV) in Specific Pathogen-Free Chicks Infected Experimentally
title_full_unstemmed Molecular Characterization and Pathogenicity of Chicken Parvovirus (ChPV) in Specific Pathogen-Free Chicks Infected Experimentally
title_short Molecular Characterization and Pathogenicity of Chicken Parvovirus (ChPV) in Specific Pathogen-Free Chicks Infected Experimentally
title_sort molecular characterization and pathogenicity of chicken parvovirus (chpv) in specific pathogen-free chicks infected experimentally
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459526/
https://www.ncbi.nlm.nih.gov/pubmed/32722416
http://dx.doi.org/10.3390/pathogens9080606
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