Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows

SIMPLE SUMMARY: Bovine embryos are nowadays produced in laboratories, frozen and transferred to other cows. However, the percentage of pregnancies obtained after these transfers as well as difficulties found during labor, especially due to increased size of calves, are a matter of great concern. One of the possible explanations for these problems relies on the embryo being produced in in vitro conditions (laboratory settings), more specifically the culture medium (liquid) used to develop these embryos. In an attempt to better mimic what happens naturally, female reproductive liquids (from oviducts and uterus) were used as a supplement to the culture of the embryos. As controls, embryos produced using the standard protocol in the laboratory were produced, as well as embryos derived from artificial insemination of cows (in vivo). An evaluation on the pregnancy rates, how the hormonal profile of the recipients changed during pregnancy, difficulties during parturitions, and phenotype of calves were recorded. Results showed that all the groups were very similar, but many differences were noted on the hormonal profiles during pregnancy. In conclusion, all systems provided safe production of calves, but long-term analysis of these calves is necessary to understand the future impact of the laboratory protocols. ABSTRACT: The increasing use of in vitro embryo production (IVP) followed by embryo transfer (ET), alongside with cryopreservation of embryos, has risen concerns regarding the possible altered pregnancy rates, calving or even neonatal mortality. One of the hypotheses for these alterations is the current culture conditions of the IVP. In an attempt to better mimic the physiological milieu, embryos were produced with female reproductive fluids (RF) as supplements to culture medium, and another group of embryos were supplemented with bovine serum albumin (BSA) as in vitro control. Embryos were cryopreserved and transferred while, in parallel, an in vivo control (artificial insemination, AI) with the same bull used for IVP was included. An overview on pregnancy rates, recipients’ hormonal levels, parturition, and resulting calves were recorded. Results show much similarity between groups in terms of pregnancy rates, gestation length and calves’ weight. Nonetheless, several differences on hormonal levels were noted between recipients carrying AI embryos especially when compared to BSA. Some calving issues and neonatal mortality were observed in both IVP groups. In conclusion, most of the parameters studied were similar between both types of IVP derived embryos and the in vivo-derived embryos, suggesting that the IVP technology used was efficient enough for the safe production of calves.