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Antibacterial Activity of Sulfated Galactans from Eucheuma serra and Gracilari verrucosa against Diarrheagenic Escherichia coli via the Disruption of the Cell Membrane Structure

Seaweed sulfated polysaccharides have attracted significant attention due to their antibacterial activity. This work investigated the antibacterial activity and mechanism of depolymerized sulfated galactans from Eucheuma serra (E. serra) and Gracilaria verrucosa (G. verrucosa) against enterotoxigeni...

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Autores principales: Liu, Yixiang, Ma, Yu, Chen, Zhaohua, Li, Donghui, Liu, Wenqiang, Huang, Ling, Zou, Chao, Cao, Min-Jie, Liu, Guang-Ming, Wang, Yanbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459719/
https://www.ncbi.nlm.nih.gov/pubmed/32751049
http://dx.doi.org/10.3390/md18080397
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author Liu, Yixiang
Ma, Yu
Chen, Zhaohua
Li, Donghui
Liu, Wenqiang
Huang, Ling
Zou, Chao
Cao, Min-Jie
Liu, Guang-Ming
Wang, Yanbo
author_facet Liu, Yixiang
Ma, Yu
Chen, Zhaohua
Li, Donghui
Liu, Wenqiang
Huang, Ling
Zou, Chao
Cao, Min-Jie
Liu, Guang-Ming
Wang, Yanbo
author_sort Liu, Yixiang
collection PubMed
description Seaweed sulfated polysaccharides have attracted significant attention due to their antibacterial activity. This work investigated the antibacterial activity and mechanism of depolymerized sulfated galactans from Eucheuma serra (E. serra) and Gracilaria verrucosa (G. verrucosa) against enterotoxigenic Escherichia coli (ETEC) K88. The results show that removing the metal ions improves the anti-ETEC K88 activity of the galactans. The fluorescence labeling study confirmed that the sulfated galactans penetrated the cell walls and eventually reached the interior of the ETEC K88. Nucleic acid staining and intracellular protein leakage were also observed, indicating the destruction of permeability and integrity of the cell membrane. Interestingly, the two polysaccharides exhibited no effect on the proliferation of the selected Gram-positive bacteria and yeast. This indicates that the cell wall structure of the microorganisms could influence the bacteriostatic activity of the sulfated polysaccharides, as well. These results suggest that the sulfated seaweed polysaccharides might have potential application value in antibacterial diarrhea.
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spelling pubmed-74597192020-09-02 Antibacterial Activity of Sulfated Galactans from Eucheuma serra and Gracilari verrucosa against Diarrheagenic Escherichia coli via the Disruption of the Cell Membrane Structure Liu, Yixiang Ma, Yu Chen, Zhaohua Li, Donghui Liu, Wenqiang Huang, Ling Zou, Chao Cao, Min-Jie Liu, Guang-Ming Wang, Yanbo Mar Drugs Article Seaweed sulfated polysaccharides have attracted significant attention due to their antibacterial activity. This work investigated the antibacterial activity and mechanism of depolymerized sulfated galactans from Eucheuma serra (E. serra) and Gracilaria verrucosa (G. verrucosa) against enterotoxigenic Escherichia coli (ETEC) K88. The results show that removing the metal ions improves the anti-ETEC K88 activity of the galactans. The fluorescence labeling study confirmed that the sulfated galactans penetrated the cell walls and eventually reached the interior of the ETEC K88. Nucleic acid staining and intracellular protein leakage were also observed, indicating the destruction of permeability and integrity of the cell membrane. Interestingly, the two polysaccharides exhibited no effect on the proliferation of the selected Gram-positive bacteria and yeast. This indicates that the cell wall structure of the microorganisms could influence the bacteriostatic activity of the sulfated polysaccharides, as well. These results suggest that the sulfated seaweed polysaccharides might have potential application value in antibacterial diarrhea. MDPI 2020-07-29 /pmc/articles/PMC7459719/ /pubmed/32751049 http://dx.doi.org/10.3390/md18080397 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Yixiang
Ma, Yu
Chen, Zhaohua
Li, Donghui
Liu, Wenqiang
Huang, Ling
Zou, Chao
Cao, Min-Jie
Liu, Guang-Ming
Wang, Yanbo
Antibacterial Activity of Sulfated Galactans from Eucheuma serra and Gracilari verrucosa against Diarrheagenic Escherichia coli via the Disruption of the Cell Membrane Structure
title Antibacterial Activity of Sulfated Galactans from Eucheuma serra and Gracilari verrucosa against Diarrheagenic Escherichia coli via the Disruption of the Cell Membrane Structure
title_full Antibacterial Activity of Sulfated Galactans from Eucheuma serra and Gracilari verrucosa against Diarrheagenic Escherichia coli via the Disruption of the Cell Membrane Structure
title_fullStr Antibacterial Activity of Sulfated Galactans from Eucheuma serra and Gracilari verrucosa against Diarrheagenic Escherichia coli via the Disruption of the Cell Membrane Structure
title_full_unstemmed Antibacterial Activity of Sulfated Galactans from Eucheuma serra and Gracilari verrucosa against Diarrheagenic Escherichia coli via the Disruption of the Cell Membrane Structure
title_short Antibacterial Activity of Sulfated Galactans from Eucheuma serra and Gracilari verrucosa against Diarrheagenic Escherichia coli via the Disruption of the Cell Membrane Structure
title_sort antibacterial activity of sulfated galactans from eucheuma serra and gracilari verrucosa against diarrheagenic escherichia coli via the disruption of the cell membrane structure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459719/
https://www.ncbi.nlm.nih.gov/pubmed/32751049
http://dx.doi.org/10.3390/md18080397
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