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The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study
The modification of the microbiome through fecal microbiota transplantation (FMT) is becoming a very promising therapeutic option for inflammatory bowel disease (IBD) patients. Our pilot study aimed to assess the effectiveness of multi-session FMT treatment in active ulcerative colitis (UC) patients...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459721/ https://www.ncbi.nlm.nih.gov/pubmed/32756350 http://dx.doi.org/10.3390/biomedicines8080268 |
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author | Mańkowska-Wierzbicka, Dorota Stelmach-Mardas, Marta Gabryel, Marcin Tomczak, Hanna Skrzypczak-Zielińska, Marzena Zakerska-Banaszak, Oliwia Sowińska, Anna Mahadea, Dagmara Baturo, Alina Wolko, Łukasz Słomski, Ryszard Dobrowolska, Agnieszka |
author_facet | Mańkowska-Wierzbicka, Dorota Stelmach-Mardas, Marta Gabryel, Marcin Tomczak, Hanna Skrzypczak-Zielińska, Marzena Zakerska-Banaszak, Oliwia Sowińska, Anna Mahadea, Dagmara Baturo, Alina Wolko, Łukasz Słomski, Ryszard Dobrowolska, Agnieszka |
author_sort | Mańkowska-Wierzbicka, Dorota |
collection | PubMed |
description | The modification of the microbiome through fecal microbiota transplantation (FMT) is becoming a very promising therapeutic option for inflammatory bowel disease (IBD) patients. Our pilot study aimed to assess the effectiveness of multi-session FMT treatment in active ulcerative colitis (UC) patients. Ten patients with UC were treated with multi-session FMT (200 mL) from healthy donors, via colonoscopy/gastroscopy. Patients were evaluated as follows: at baseline, at week 7, and after 6 months, routine blood tests (including C reactive protein (CRP) and calprotectin) were performed. 16S rRNA gene (V3V4) sequencing was used for metagenomic analysis. The severity of UC was classified based on the Truelove–Witts index. The assessment of microbial diversity showed significant differences between recipients and healthy donors. FMT contributed to long-term, significant clinical and biochemical improvement. Metagenomic analysis revealed an increase in the amount of Lactobacillaceaea, Micrococcaceae, Prevotellaceae, and TM7 phylumsp.oral clone EW055 during FMT, whereas Staphylococcaceae and Bacillaceae declined significantly. A positive increase in the proportion of the genera Bifidobacterium, Lactobacillus, Rothia, Streptococcus, and Veillonella and a decrease in Bacillus, Bacteroides, and Staphylococcus were observed based on the correlation between calprotectin and Bacillus and Staphylococcus; ferritin and Lactobacillus, Veillonella, and Bifidobacterium abundance was indicated. A positive change in the abundance of Firmicutes was observed during FMT and after 6 months. The application of multi-session FMT led to the restoration of recipients’ microbiota and resulted in the remission of patients with active UC. |
format | Online Article Text |
id | pubmed-7459721 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74597212020-09-02 The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study Mańkowska-Wierzbicka, Dorota Stelmach-Mardas, Marta Gabryel, Marcin Tomczak, Hanna Skrzypczak-Zielińska, Marzena Zakerska-Banaszak, Oliwia Sowińska, Anna Mahadea, Dagmara Baturo, Alina Wolko, Łukasz Słomski, Ryszard Dobrowolska, Agnieszka Biomedicines Article The modification of the microbiome through fecal microbiota transplantation (FMT) is becoming a very promising therapeutic option for inflammatory bowel disease (IBD) patients. Our pilot study aimed to assess the effectiveness of multi-session FMT treatment in active ulcerative colitis (UC) patients. Ten patients with UC were treated with multi-session FMT (200 mL) from healthy donors, via colonoscopy/gastroscopy. Patients were evaluated as follows: at baseline, at week 7, and after 6 months, routine blood tests (including C reactive protein (CRP) and calprotectin) were performed. 16S rRNA gene (V3V4) sequencing was used for metagenomic analysis. The severity of UC was classified based on the Truelove–Witts index. The assessment of microbial diversity showed significant differences between recipients and healthy donors. FMT contributed to long-term, significant clinical and biochemical improvement. Metagenomic analysis revealed an increase in the amount of Lactobacillaceaea, Micrococcaceae, Prevotellaceae, and TM7 phylumsp.oral clone EW055 during FMT, whereas Staphylococcaceae and Bacillaceae declined significantly. A positive increase in the proportion of the genera Bifidobacterium, Lactobacillus, Rothia, Streptococcus, and Veillonella and a decrease in Bacillus, Bacteroides, and Staphylococcus were observed based on the correlation between calprotectin and Bacillus and Staphylococcus; ferritin and Lactobacillus, Veillonella, and Bifidobacterium abundance was indicated. A positive change in the abundance of Firmicutes was observed during FMT and after 6 months. The application of multi-session FMT led to the restoration of recipients’ microbiota and resulted in the remission of patients with active UC. MDPI 2020-08-03 /pmc/articles/PMC7459721/ /pubmed/32756350 http://dx.doi.org/10.3390/biomedicines8080268 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mańkowska-Wierzbicka, Dorota Stelmach-Mardas, Marta Gabryel, Marcin Tomczak, Hanna Skrzypczak-Zielińska, Marzena Zakerska-Banaszak, Oliwia Sowińska, Anna Mahadea, Dagmara Baturo, Alina Wolko, Łukasz Słomski, Ryszard Dobrowolska, Agnieszka The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study |
title | The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study |
title_full | The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study |
title_fullStr | The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study |
title_full_unstemmed | The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study |
title_short | The Effectiveness of Multi-Session FMT Treatment in Active Ulcerative Colitis Patients: A Pilot Study |
title_sort | effectiveness of multi-session fmt treatment in active ulcerative colitis patients: a pilot study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459721/ https://www.ncbi.nlm.nih.gov/pubmed/32756350 http://dx.doi.org/10.3390/biomedicines8080268 |
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