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Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon

Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antivi...

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Autores principales: Cárdenas, Constanza, Guzmán, Fanny, Carmona, Marisela, Muñoz, Cristian, Nilo, Luis, Labra, Alvaro, Marshall, Sergio H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459813/
https://www.ncbi.nlm.nih.gov/pubmed/32717804
http://dx.doi.org/10.3390/pathogens9080600
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author Cárdenas, Constanza
Guzmán, Fanny
Carmona, Marisela
Muñoz, Cristian
Nilo, Luis
Labra, Alvaro
Marshall, Sergio H.
author_facet Cárdenas, Constanza
Guzmán, Fanny
Carmona, Marisela
Muñoz, Cristian
Nilo, Luis
Labra, Alvaro
Marshall, Sergio H.
author_sort Cárdenas, Constanza
collection PubMed
description Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using Salmo salar fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture.
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spelling pubmed-74598132020-09-02 Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon Cárdenas, Constanza Guzmán, Fanny Carmona, Marisela Muñoz, Cristian Nilo, Luis Labra, Alvaro Marshall, Sergio H. Pathogens Article Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using Salmo salar fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture. MDPI 2020-07-23 /pmc/articles/PMC7459813/ /pubmed/32717804 http://dx.doi.org/10.3390/pathogens9080600 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cárdenas, Constanza
Guzmán, Fanny
Carmona, Marisela
Muñoz, Cristian
Nilo, Luis
Labra, Alvaro
Marshall, Sergio H.
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon
title Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon
title_full Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon
title_fullStr Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon
title_full_unstemmed Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon
title_short Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon
title_sort synthetic peptides as a promising alternative to control viral infections in atlantic salmon
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459813/
https://www.ncbi.nlm.nih.gov/pubmed/32717804
http://dx.doi.org/10.3390/pathogens9080600
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