Cargando…
ARDEP, a Rapid Degenerate Primer Design Pipeline Based on k-mers for Amplicon Microbiome Studies
The survey of microbial diversity in various environments has relied upon the widespread use of well-evaluated amplification primers for taxonomic marker genes (e.g., prokaryotic 16S and fungal ITS). However, it is urgent to develop a fast and accurate bioinformatic program to design primers for mic...
Autores principales: | Wu, Yueni, Feng, Kai, Wei, Ziyan, Wang, Zhujun, Deng, Ye |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459862/ https://www.ncbi.nlm.nih.gov/pubmed/32824566 http://dx.doi.org/10.3390/ijerph17165958 |
Ejemplares similares
-
Slippage of degenerate primers can cause variation in amplicon length
por: Elbrecht, Vasco, et al.
Publicado: (2018) -
Skip the Alignment: Degenerate, Multiplex Primer and Probe Design Using K-mer Matching Instead of Alignments
por: Hysom, David A., et al.
Publicado: (2012) -
Amplicon and Metagenomic Analysis of Middle East Respiratory Syndrome (MERS) Coronavirus and the Microbiome in Patients with Severe MERS
por: Aljabr, Waleed, et al.
Publicado: (2021) -
Simulate_PCR for amplicon prediction and annotation from multiplex, degenerate primers and probes
por: Gardner, Shea N, et al.
Publicado: (2014) -
Habitat environments impacted the gut microbiome of long-distance migratory swan geese but central species conserved
por: Wu, Yueni, et al.
Publicado: (2018)