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Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses
Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PG...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460212/ https://www.ncbi.nlm.nih.gov/pubmed/32784428 http://dx.doi.org/10.3390/ijerph17165740 |
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author | Larson, Camille Opichka, Megan McGlynn, Mark L. Collins, Christopher W. Slivka, Dustin |
author_facet | Larson, Camille Opichka, Megan McGlynn, Mark L. Collins, Christopher W. Slivka, Dustin |
author_sort | Larson, Camille |
collection | PubMed |
description | Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a, PGC-1α-b, and PGC-1α-c. Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention (p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α (p = 0.028) and its truncated isoform, NT-PGC-1α (p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise- and temperature-induced PGC-1α response. |
format | Online Article Text |
id | pubmed-7460212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74602122020-09-02 Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses Larson, Camille Opichka, Megan McGlynn, Mark L. Collins, Christopher W. Slivka, Dustin Int J Environ Res Public Health Article Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a, PGC-1α-b, and PGC-1α-c. Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention (p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α (p = 0.028) and its truncated isoform, NT-PGC-1α (p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise- and temperature-induced PGC-1α response. MDPI 2020-08-08 2020-08 /pmc/articles/PMC7460212/ /pubmed/32784428 http://dx.doi.org/10.3390/ijerph17165740 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Larson, Camille Opichka, Megan McGlynn, Mark L. Collins, Christopher W. Slivka, Dustin Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses |
title | Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses |
title_full | Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses |
title_fullStr | Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses |
title_full_unstemmed | Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses |
title_short | Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses |
title_sort | exercise- and cold-induced human pgc-1α mrna isoform specific responses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460212/ https://www.ncbi.nlm.nih.gov/pubmed/32784428 http://dx.doi.org/10.3390/ijerph17165740 |
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