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Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses

Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PG...

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Autores principales: Larson, Camille, Opichka, Megan, McGlynn, Mark L., Collins, Christopher W., Slivka, Dustin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460212/
https://www.ncbi.nlm.nih.gov/pubmed/32784428
http://dx.doi.org/10.3390/ijerph17165740
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author Larson, Camille
Opichka, Megan
McGlynn, Mark L.
Collins, Christopher W.
Slivka, Dustin
author_facet Larson, Camille
Opichka, Megan
McGlynn, Mark L.
Collins, Christopher W.
Slivka, Dustin
author_sort Larson, Camille
collection PubMed
description Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a, PGC-1α-b, and PGC-1α-c. Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention (p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α (p = 0.028) and its truncated isoform, NT-PGC-1α (p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise- and temperature-induced PGC-1α response.
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spelling pubmed-74602122020-09-02 Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses Larson, Camille Opichka, Megan McGlynn, Mark L. Collins, Christopher W. Slivka, Dustin Int J Environ Res Public Health Article Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α, the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α, which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a, PGC-1α-b, and PGC-1α-c. Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention (p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α (p = 0.028) and its truncated isoform, NT-PGC-1α (p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise- and temperature-induced PGC-1α response. MDPI 2020-08-08 2020-08 /pmc/articles/PMC7460212/ /pubmed/32784428 http://dx.doi.org/10.3390/ijerph17165740 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Larson, Camille
Opichka, Megan
McGlynn, Mark L.
Collins, Christopher W.
Slivka, Dustin
Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses
title Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses
title_full Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses
title_fullStr Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses
title_full_unstemmed Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses
title_short Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses
title_sort exercise- and cold-induced human pgc-1α mrna isoform specific responses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460212/
https://www.ncbi.nlm.nih.gov/pubmed/32784428
http://dx.doi.org/10.3390/ijerph17165740
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