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Contact Effect of a Methylobacterium sp. Extract on Biofilm of a Mycobacterium chimaera Strain Isolated from a 3T Heater-Cooler System
Mycobacterium chimaera is an opportunistic slowly growing non-tuberculous mycobacteriumof increasing importance due to the outbreak of cases associated with contaminated 3T heater-cooler device (HCD) extracorporeal membrane oxygenator (ECMO). The aim of this study was to evaluate the effect of pre-t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460266/ https://www.ncbi.nlm.nih.gov/pubmed/32756304 http://dx.doi.org/10.3390/antibiotics9080474 |
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author | Pradal, Inés Esteban, Jaime Mediero, Arancha García-Coca, Marta Aguilera-Correa, John Jairo |
author_facet | Pradal, Inés Esteban, Jaime Mediero, Arancha García-Coca, Marta Aguilera-Correa, John Jairo |
author_sort | Pradal, Inés |
collection | PubMed |
description | Mycobacterium chimaera is an opportunistic slowly growing non-tuberculous mycobacteriumof increasing importance due to the outbreak of cases associated with contaminated 3T heater-cooler device (HCD) extracorporeal membrane oxygenator (ECMO). The aim of this study was to evaluate the effect of pre-treating a surface with a Methylobacterium sp. CECT 7180 extract to inhibit the M. chimaera ECMO biofilm as well as of the treatment after different dehydration times. Surface adherence, biofilm formation and treatment effect were evaluated by estimating colony-forming units (CFU) per square centimeter and characterizing the amount of covered surface area, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. We found that exposing a surface to the Methylobacterium sp. CECT 7180 extract inhibited M. chimaera ECMO biofilm development. This effect could be result of the effect of Methylobacterium proteins, such as DNaK, trigger factor, and xanthine oxidase. In conclusion, exposing a surface to the Methylobacterium sp. extract inhibits M. chimaera ECMO biofilm development. Furthermore, this extract could be used as a pre-treatment prior to disinfection protocols for equipment contaminated with mycobacteria after dehydration for at least 96 h. |
format | Online Article Text |
id | pubmed-7460266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74602662020-09-02 Contact Effect of a Methylobacterium sp. Extract on Biofilm of a Mycobacterium chimaera Strain Isolated from a 3T Heater-Cooler System Pradal, Inés Esteban, Jaime Mediero, Arancha García-Coca, Marta Aguilera-Correa, John Jairo Antibiotics (Basel) Article Mycobacterium chimaera is an opportunistic slowly growing non-tuberculous mycobacteriumof increasing importance due to the outbreak of cases associated with contaminated 3T heater-cooler device (HCD) extracorporeal membrane oxygenator (ECMO). The aim of this study was to evaluate the effect of pre-treating a surface with a Methylobacterium sp. CECT 7180 extract to inhibit the M. chimaera ECMO biofilm as well as of the treatment after different dehydration times. Surface adherence, biofilm formation and treatment effect were evaluated by estimating colony-forming units (CFU) per square centimeter and characterizing the amount of covered surface area, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. We found that exposing a surface to the Methylobacterium sp. CECT 7180 extract inhibited M. chimaera ECMO biofilm development. This effect could be result of the effect of Methylobacterium proteins, such as DNaK, trigger factor, and xanthine oxidase. In conclusion, exposing a surface to the Methylobacterium sp. extract inhibits M. chimaera ECMO biofilm development. Furthermore, this extract could be used as a pre-treatment prior to disinfection protocols for equipment contaminated with mycobacteria after dehydration for at least 96 h. MDPI 2020-08-03 /pmc/articles/PMC7460266/ /pubmed/32756304 http://dx.doi.org/10.3390/antibiotics9080474 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pradal, Inés Esteban, Jaime Mediero, Arancha García-Coca, Marta Aguilera-Correa, John Jairo Contact Effect of a Methylobacterium sp. Extract on Biofilm of a Mycobacterium chimaera Strain Isolated from a 3T Heater-Cooler System |
title | Contact Effect of a Methylobacterium
sp. Extract on Biofilm of a Mycobacterium
chimaera Strain Isolated from a 3T Heater-Cooler System |
title_full | Contact Effect of a Methylobacterium
sp. Extract on Biofilm of a Mycobacterium
chimaera Strain Isolated from a 3T Heater-Cooler System |
title_fullStr | Contact Effect of a Methylobacterium
sp. Extract on Biofilm of a Mycobacterium
chimaera Strain Isolated from a 3T Heater-Cooler System |
title_full_unstemmed | Contact Effect of a Methylobacterium
sp. Extract on Biofilm of a Mycobacterium
chimaera Strain Isolated from a 3T Heater-Cooler System |
title_short | Contact Effect of a Methylobacterium
sp. Extract on Biofilm of a Mycobacterium
chimaera Strain Isolated from a 3T Heater-Cooler System |
title_sort | contact effect of a methylobacterium
sp. extract on biofilm of a mycobacterium
chimaera strain isolated from a 3t heater-cooler system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460266/ https://www.ncbi.nlm.nih.gov/pubmed/32756304 http://dx.doi.org/10.3390/antibiotics9080474 |
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