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Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection

The population of brown trout (Salmo trutta fario) in continental Europe is on the decline, with infectious diseases confirmed as one of the causative factors. However, no data on the epizootiological situation of wild fish in the Czech Republic are currently available. In this study, brown trout (n...

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Autores principales: Pojezdal, Ľubomír, Adamek, Mikolaj, Syrová, Eva, Steinhagen, Dieter, Minářová, Hana, Papežíková, Ivana, Seidlová, Veronika, Reschová, Stanislava, Palíková, Miroslava
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460431/
https://www.ncbi.nlm.nih.gov/pubmed/32722219
http://dx.doi.org/10.3390/pathogens9080604
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author Pojezdal, Ľubomír
Adamek, Mikolaj
Syrová, Eva
Steinhagen, Dieter
Minářová, Hana
Papežíková, Ivana
Seidlová, Veronika
Reschová, Stanislava
Palíková, Miroslava
author_facet Pojezdal, Ľubomír
Adamek, Mikolaj
Syrová, Eva
Steinhagen, Dieter
Minářová, Hana
Papežíková, Ivana
Seidlová, Veronika
Reschová, Stanislava
Palíková, Miroslava
author_sort Pojezdal, Ľubomír
collection PubMed
description The population of brown trout (Salmo trutta fario) in continental Europe is on the decline, with infectious diseases confirmed as one of the causative factors. However, no data on the epizootiological situation of wild fish in the Czech Republic are currently available. In this study, brown trout (n = 260) from eight rivers were examined for the presence of viral and parasitical pathogens. Salmonid alphavirus-2, infectious pancreatic necrosis virus, piscine novirhabdovirus (VHSV) and salmonid novirhabdovirus (IHNV) were not detected using PCR. Cell culturing showed no viruses as well, and serological analysis of 110 sera did not detect any specific antibodies against VHSV or IHNV. Fish from two rivers were positive for the presence of piscine orthoreovirus-3 (PRV-3), subtype PRV-3b. However, none of the PRV-3-positive fish showed gross pathologies typically associated with PRV infections. By far the most widespread pathogen was Tetracapsuloides bryosalmonae which was confirmed in each of the examined locations, with a prevalence of up to 65% and 100%, as established by immunohistochemistry and PCR, respectively. Furthermore, up to 43.8% of fish showed signs of proliferative kidney disease caused by T. bryosalmonae, suggesting that this parasite is a main health challenge for brown trout in the Czech Republic.
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spelling pubmed-74604312020-09-03 Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection Pojezdal, Ľubomír Adamek, Mikolaj Syrová, Eva Steinhagen, Dieter Minářová, Hana Papežíková, Ivana Seidlová, Veronika Reschová, Stanislava Palíková, Miroslava Pathogens Article The population of brown trout (Salmo trutta fario) in continental Europe is on the decline, with infectious diseases confirmed as one of the causative factors. However, no data on the epizootiological situation of wild fish in the Czech Republic are currently available. In this study, brown trout (n = 260) from eight rivers were examined for the presence of viral and parasitical pathogens. Salmonid alphavirus-2, infectious pancreatic necrosis virus, piscine novirhabdovirus (VHSV) and salmonid novirhabdovirus (IHNV) were not detected using PCR. Cell culturing showed no viruses as well, and serological analysis of 110 sera did not detect any specific antibodies against VHSV or IHNV. Fish from two rivers were positive for the presence of piscine orthoreovirus-3 (PRV-3), subtype PRV-3b. However, none of the PRV-3-positive fish showed gross pathologies typically associated with PRV infections. By far the most widespread pathogen was Tetracapsuloides bryosalmonae which was confirmed in each of the examined locations, with a prevalence of up to 65% and 100%, as established by immunohistochemistry and PCR, respectively. Furthermore, up to 43.8% of fish showed signs of proliferative kidney disease caused by T. bryosalmonae, suggesting that this parasite is a main health challenge for brown trout in the Czech Republic. MDPI 2020-07-24 /pmc/articles/PMC7460431/ /pubmed/32722219 http://dx.doi.org/10.3390/pathogens9080604 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pojezdal, Ľubomír
Adamek, Mikolaj
Syrová, Eva
Steinhagen, Dieter
Minářová, Hana
Papežíková, Ivana
Seidlová, Veronika
Reschová, Stanislava
Palíková, Miroslava
Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection
title Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection
title_full Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection
title_fullStr Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection
title_full_unstemmed Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection
title_short Health Surveillance of Wild Brown Trout (Salmo trutta fario) in the Czech Republic Revealed a Coexistence of Proliferative Kidney Disease and Piscine Orthoreovirus-3 Infection
title_sort health surveillance of wild brown trout (salmo trutta fario) in the czech republic revealed a coexistence of proliferative kidney disease and piscine orthoreovirus-3 infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460431/
https://www.ncbi.nlm.nih.gov/pubmed/32722219
http://dx.doi.org/10.3390/pathogens9080604
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