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Topical Application of Phlorotannins from Brown Seaweed Mitigates Radiation Dermatitis in a Mouse Model

Radiation dermatitis (RD) is one of the most common side effects of radiotherapy; its symptoms progress from erythema to dry and moist desquamation, leading to the deterioration of the patients’ quality of life. Active metabolites in brown seaweed, including phlorotannins (PTNs), show anti-inflammat...

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Autores principales: Yang, Kyungmi, Kim, Shin-Yeong, Park, Ji-Hye, Ahn, Won-Gyun, Jung, Sang Hoon, Oh, Dongruyl, Park, Hee Chul, Choi, Changhoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460453/
https://www.ncbi.nlm.nih.gov/pubmed/32707897
http://dx.doi.org/10.3390/md18080377
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author Yang, Kyungmi
Kim, Shin-Yeong
Park, Ji-Hye
Ahn, Won-Gyun
Jung, Sang Hoon
Oh, Dongruyl
Park, Hee Chul
Choi, Changhoon
author_facet Yang, Kyungmi
Kim, Shin-Yeong
Park, Ji-Hye
Ahn, Won-Gyun
Jung, Sang Hoon
Oh, Dongruyl
Park, Hee Chul
Choi, Changhoon
author_sort Yang, Kyungmi
collection PubMed
description Radiation dermatitis (RD) is one of the most common side effects of radiotherapy; its symptoms progress from erythema to dry and moist desquamation, leading to the deterioration of the patients’ quality of life. Active metabolites in brown seaweed, including phlorotannins (PTNs), show anti-inflammatory activities; however, their medical use is limited. Here, we investigated the effects of PTNs in a mouse model of RD in vivo. X-rays (36 Gy) were delivered in three fractions to the hind legs of BALB/c mice. Macroscopic RD scoring revealed that PTNs significantly mitigated RD compared with the vehicle control. Histopathological analyses of skin tissues revealed that PTNs decreased epidermal and dermal thickness compared with the vehicle control. Western blotting indicated that PTNs augmented nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) pathway activation but attenuated radiation-induced NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) and inflammasome activation, suggesting the mitigation of acute inflammation in irradiated mouse skin. PTNs also facilitated fast recovery, as indicated by increased aquaporin 3 expression and decreased γH2AX (histone family member X) expression. Our results indicate that topical PTN application may alleviate RD symptoms by suppressing oxidative stress and inflammatory signaling and by promoting the healing process. Therefore, PTNs may show great potential as cosmeceuticals for patients with cancer suffering from radiation-induced inflammatory side effects such as RD.
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spelling pubmed-74604532020-09-03 Topical Application of Phlorotannins from Brown Seaweed Mitigates Radiation Dermatitis in a Mouse Model Yang, Kyungmi Kim, Shin-Yeong Park, Ji-Hye Ahn, Won-Gyun Jung, Sang Hoon Oh, Dongruyl Park, Hee Chul Choi, Changhoon Mar Drugs Article Radiation dermatitis (RD) is one of the most common side effects of radiotherapy; its symptoms progress from erythema to dry and moist desquamation, leading to the deterioration of the patients’ quality of life. Active metabolites in brown seaweed, including phlorotannins (PTNs), show anti-inflammatory activities; however, their medical use is limited. Here, we investigated the effects of PTNs in a mouse model of RD in vivo. X-rays (36 Gy) were delivered in three fractions to the hind legs of BALB/c mice. Macroscopic RD scoring revealed that PTNs significantly mitigated RD compared with the vehicle control. Histopathological analyses of skin tissues revealed that PTNs decreased epidermal and dermal thickness compared with the vehicle control. Western blotting indicated that PTNs augmented nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) pathway activation but attenuated radiation-induced NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) and inflammasome activation, suggesting the mitigation of acute inflammation in irradiated mouse skin. PTNs also facilitated fast recovery, as indicated by increased aquaporin 3 expression and decreased γH2AX (histone family member X) expression. Our results indicate that topical PTN application may alleviate RD symptoms by suppressing oxidative stress and inflammatory signaling and by promoting the healing process. Therefore, PTNs may show great potential as cosmeceuticals for patients with cancer suffering from radiation-induced inflammatory side effects such as RD. MDPI 2020-07-22 /pmc/articles/PMC7460453/ /pubmed/32707897 http://dx.doi.org/10.3390/md18080377 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Kyungmi
Kim, Shin-Yeong
Park, Ji-Hye
Ahn, Won-Gyun
Jung, Sang Hoon
Oh, Dongruyl
Park, Hee Chul
Choi, Changhoon
Topical Application of Phlorotannins from Brown Seaweed Mitigates Radiation Dermatitis in a Mouse Model
title Topical Application of Phlorotannins from Brown Seaweed Mitigates Radiation Dermatitis in a Mouse Model
title_full Topical Application of Phlorotannins from Brown Seaweed Mitigates Radiation Dermatitis in a Mouse Model
title_fullStr Topical Application of Phlorotannins from Brown Seaweed Mitigates Radiation Dermatitis in a Mouse Model
title_full_unstemmed Topical Application of Phlorotannins from Brown Seaweed Mitigates Radiation Dermatitis in a Mouse Model
title_short Topical Application of Phlorotannins from Brown Seaweed Mitigates Radiation Dermatitis in a Mouse Model
title_sort topical application of phlorotannins from brown seaweed mitigates radiation dermatitis in a mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7460453/
https://www.ncbi.nlm.nih.gov/pubmed/32707897
http://dx.doi.org/10.3390/md18080377
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